Enolase-2 Antibody

• 产品编号：CST-9536S      品牌：CST       原厂货号：9536S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

烯醇酶2抗体用于检测内源性烯醇酶2总蛋白水平。通过用与人烯醇酶2序列相同的合成肽免疫动物制备多克隆抗体。多抗的纯化采用蛋白酶A和肽段亲和层析的方法。烯醇酶2是一种在糖酵解过程中发挥作用的酶，负责将2-磷酸甘油酸转化成磷酸烯醇式丙酮酸(1)。哺乳类的烯醇酶有三种亚基：α, β, 和γ，它们可以形成 同源二聚体或异源二聚体。γ亚基的同源二聚体式烯醇酶是神经元特异性的(2)。临床研究发现在神经母细胞瘤(2)和小细胞肺癌(3,4)中存在着神经特异性烯 醇酶2水平的升高。因此，烯醇酶2通常在临床实验中用作疾病诊断标志物。

1. Van Obberghen, E. et al. (1988) J Neurosci Res 19, 450-6.
2. Pancholi, V. (2001) Cell Mol Life Sci 58, 902-20.
3. Stern, P. et al. (2007) Tumour Biol 28, 84-92.
4. O'Shea, P. et al. (1995) Ir J Med Sci 164, 31-6.

原厂资料：

Specificity / Sensitivity

Enolase-2 Antibody detects endogenous levels of total enolase-2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human enolase-2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Enolase is a glycolytic enzyme that is involved in the conversion of 2-phosphoglycerate to phosphoenolpyruvate (1). Mammalian enolase has three subunits: α, β, and γ, that can form homo and heterodimers. Homodimers of γ enolase are neuronal-specific (2). Clinical studies have shown elevated levels of neuro-specific enolase-2 in neuroblastoma (2) and small-cell lung cancer (3,4). Hence, enolase-2 is widely used as a diagnostic marker in a variety of clinical assays (3,4).

1. Van Obberghen, E. et al. (1988) J Neurosci Res 19, 450-6.
2. Pancholi, V. (2001) Cell Mol Life Sci 58, 902-20.
3. Stern, P. et al. (2007) Tumour Biol 28, 84-92.
4. O'Shea, P. et al. (1995) Ir J Med Sci 164, 31-6.

注意事项：

Storage: Supplied in 10mM HEPES (pH 7.5), 150 mM NaCl,
100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot
the antibody.