PDCD4 (D29C6) XP? Rabbit mAb

• 产品编号：CST-9535S      品牌：CST       原厂货号：9535S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

该单克隆抗体能够检测内源PDCD4总蛋白。该单克隆抗体是用合成的人PDCD4蛋白相应多肽免疫动物而生产制备。PDCD4（程序性死亡蛋白4）被鉴定为致瘤性转化的抑制因子（1）。它可与真核启动子eIF4AI 和 eIF4AII 相互作用(2)。PDCD4与elF4A共定位于胞浆，作为ATP 依赖性的RNA 解旋酶而抑制eIF4A的活性(2)。PDCD4 抑制CAP-依赖性的翻译（2）。在分裂素的刺激下, S6K1酶在67位丝氨酸磷酸化PDCD4，接着通过泛素化通路降解，增强转录和细胞生长。

1. Cmarik, J.L. et al. (1999) Proc Natl Acad Sci USA 96, 14037-42.
2. Yang, H.S. et al. (2003) Mol Cell Biol 23, 26-37.
3. Dorrello, N.V. et al. (2006) Science 314, 467-71.

原厂资料：

Specificity / Sensitivity

PDCD4 (D29C6) XP® Rabbit mAb detects endogenous levels of total PDCD4 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to human PDCD4 protein.

Background

PDCD4 (programmed cell death protein 4) was identified as a suppressor of neoplastic transformation (1). It interacts with eukaryotic initiation factors eIF4AI and eIF4AII (2). PDCD4 colocalizes with eIF4A in the cytoplasm and inhibits the activity of eIF4A as an ATP-dependent RNA helicase (2). PDCD4 inhibits cap-dependent translation (2). Upon stimulation by mitogens, PDCD4 is phosphorylated on Ser67 by S6K1 kinase and then degraded through the ubiquitin pathway leading to enhanced translation and cell growth (3).

1. Cmarik, J.L. et al. (1999) Proc Natl Acad Sci USA 96, 14037-42.
2. Yang, H.S. et al. (2003) Mol Cell Biol 23, 26-37.
3. Dorrello, N.V. et al. (2006) Science 314, 467-71.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody