Smad3 (C67H9) Rabbit mAb

• 产品编号：CST-9523S      品牌：CST       原厂货号：9523S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Smad3 (C67H9) Rabbit mAb兔单抗可以识别内源的总Smad3蛋白。经检测与其他家族成员没有交叉反应。合成与Smad3氨基末端氨基酸残基序列一致的肽段，免疫动物获得单克隆抗体。Smad信号转导分子家族成员是胞内通路中TGF-β信号从细胞表面传送到细胞核的重要组成部分。Smad 可被分为三类：受体调控型Smad（简称R-Smad），主要包括Smad1,2,3,5和8；共同介导型Smad（co-Smad）包括Smad4；还有拮抗型或称抑制型Smad（I-Smads），包括Smad6和Smad7[1-5]。激活的I型受体与特异的R-Smad有关，并能在R-Smad的保守的C端SSXS基序处对其磷酸化。磷酸化的R-Smad与受体分离，再与co-Smad（Smad4）形成异侧复合物，使复合物迁移入核。一旦进入核内，Smad可以各种DNA绑定蛋白为目标调控转录反应。[6-8]TGF-β刺激后，Smad2和Smad3羧基末端丝氨酸残基（Smad2的465位和467位丝氨酸，Smad3的423和425位丝氨酸）被TGF-β Receptor 1磷酸化。磷酸化的Smad2/3与Smad4形成复合物进入细胞核调控基因表达（9-11）。

1. Heldin, C.H. et al. (1997) Nature 390, 465-71.
2. Attisano, L. and Wrana, J.L. (1998) Curr Opin Cell Biol 10, 188-94.
3. Derynck, R. et al. (1998) Cell 95, 737-40.
4. Massagué, J. (1998) Annu Rev Biochem 67, 753-91.
5. Whitman, M. (1998) Genes Dev 12, 2445-62.
6. Wu, G. et al. (2000) Science 287, 92-7.
7. Attisano, L. and Wrana, J.L. (2002) Science 296, 1646-7.
8. Moustakas, A. et al. (2001) J Cell Sci 114, 4359-69.
9. Abdollah, S. et al. (1997) J. Biol. Chem. 272, 27678-27685.
10. Souchelnytskyi, S. et al. (1997) J. Biol. Chem. 272, 28107-28115.
11. Liu, X. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 10669-10674.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Xenopus, Zebrafish, Bovine

Specificity / Sensitivity

Smad3 (C67H9) Rabbit mAb detects endogenous levels of total Smad3 protein. No cross reactivity was detected with other family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues at the amino terminus of Smad3.

Background

Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 (1-5). Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses (6-8).Following stimulation by TGF-β, Smad2 and Smad3 become phosphorylated at their carboxyl termini (Ser465 and 467 on Smad2; Ser423 and 425 on Smad3) by TGF-β Receptor I. Phosphorylated Smad 2/3 can complex with Smad4, translocate to the nucleus and regulate gene expression (9-11).

1. Heldin, C.H. et al. (1997) Nature 390, 465-71.
2. Attisano, L. and Wrana, J.L. (1998) Curr Opin Cell Biol 10, 188-94.
3. Derynck, R. et al. (1998) Cell 95, 737-40.
4. Massagué, J. (1998) Annu Rev Biochem 67, 753-91.
5. Whitman, M. (1998) Genes Dev 12, 2445-62.
6. Wu, G. et al. (2000) Science 287, 92-7.
7. Attisano, L. and Wrana, J.L. (2002) Science 296, 1646-7.
8. Moustakas, A. et al. (2001) J Cell Sci 114, 4359-69.
9. Abdollah, S. et al. (1997) J. Biol. Chem. 272, 27678-27685.
10. Souchelnytskyi, S. et al. (1997) J. Biol. Chem. 272, 28107-28115.
11. Liu, X. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 10669-10674.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium
azide. Store at –20°C. Do not aliquot the antibody