Smad4 Antibody

• 产品编号：CST-9515S      品牌：Cell Signaling Technology       原厂货号：9515S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Smad4 Antibody可以识别内源性的总Smad4蛋白。合成与人Smad4的邻近 Pro278氨基酸残基序列一致的肽段，免疫动物获得多克隆抗体。抗体通过蛋白A和肽亲和层析纯化。Smad信号转导分子家族成员是胞内通路中TGF-β信号从细胞表面传送到细胞核的重要组成部分。Smad 可被分为三类：受体调控型Smad（简称R-Smad），主要包括Smad1,2,3,5和8；共同介导型Smad（co-Smad）包括Smad4；还有拮抗型或称抑制型Smad（I-Smads），包括Smad6和Smad7[1-5]。激活的I型受体与特异的R-Smad有关，并能在R-Smad的保守的C端SSXS基序处对其磷酸化。磷酸化的R-Smad与受体分离，再与co-Smad（Smad4）形成异侧复合物，使复合物迁移入核。一旦进入核内，Smad可以各种DNA绑定蛋白为目标调控转录反应。[6-8]

1. Heldin, C. H. et al. (1997) Nature 390, 465-471.
2. Attisano, L. and Wrana, J.L. (1998) Curr. Opin. Cell Biol. 10, 188-194.
3. Derynck, R. et al. (1998) Cell 95, 737-740.
4. Massague, J. (1998) Annu. Rev. Biochem. 67, 753-791.
5. Whitman, . (1998) Genes Dev. 12, 2445-2462.
6. Wrana, J. (2000) Science 23, 1-9.
7. Attisano, L. and Wrana, J. (2002) Science 296, 1646-1647.
8. Moustakas, A. et al. (2001) J. Cell Sci. 114, 4359-4369.

原厂资料：

Specificity / Sensitivity

Smad4 Antibody detects endogenous levels of total Smad4 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the residues surrounding Pro278 of human Smad4. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 (1-5). Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses (6-8).

1. Heldin, C. H. et al. (1997) Nature 390, 465-471.
2. Attisano, L. and Wrana, J.L. (1998) Curr. Opin. Cell Biol. 10, 188-194.
3. Derynck, R. et al. (1998) Cell 95, 737-740.
4. Massague, J. (1998) Annu. Rev. Biochem. 67, 753-791.
5. Whitman, . (1998) Genes Dev. 12, 2445-2462.
6. Wrana, J. (2000) Science 23, 1-9.
7. Attisano, L. and Wrana, J. (2002) Science 296, 1646-1647.
8. Moustakas, A. et al. (2001) J. Cell Sci. 114, 4359-4369.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.