APC6 (D8D8) Rabbit mAb

• 产品编号：CST-9499S      品牌：CST       原厂货号：9499S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

APC6 (D8D8) Rabbit mAb能够识别内源性APC6总蛋白。基于序列比对，该抗体预计不与APC8/CDC23和APC3/CDC27发生交叉反应。该单克隆抗体是由合成的人源的针对APC6蛋白羧基末端的肽段免疫动物生产的。所有真核细胞的增殖严格取决于细胞分裂后期促进复合体的泛素连接酶（E3）的活性，该复合体的主要功能是触发细胞周期从中期向后期过渡。 APC/C是在有丝分裂中期细胞核中发现的一种1.5 MDa的蛋白质。该复合体分散在整个细胞质并和有丝分裂过程中的纺锤体部分相联系。APC/C通过促进底物蛋白上多聚泛肽链的装配来执行其各种功能，从而靶向这些蛋白质并通过26S蛋白酶体降解（1,2）。在人类中，12个不同的APC/ C亚基已经鉴定。像所有的E3酶一样，APC/C能够利用素E1酶激活的泛素蛋白残基，然后转移到E2酶。事实上，APC/C已被证明能够瞬时与UBCH5和UBCH10 E2酶相互作用，部分通过含有APC11亚基的RING-指结构域（3-5）。除E2酶之外，APC/C的活性也严格取决于细胞周期的特定阶段联合APC/ C的几个辅助因子中的一个。这些蛋白中研究比较到位的是Cdc20 和Cdh1，其中含有C-末端的WD40结构域和参与APC/C底物的识别，通过与这些底物中的特定识别元件相互作用(6)，称为D-盒（7），以及KEN盒（8）。APC6/CDC16是APC/C肽重复序列结构域亚复合体的一个组成部分，包括APC8/CDC23和APC3/CDC27。该亚复合体被认为在协调催化模块和底物识别模块并列的过程中起到重要作用，模块与辅助活化剂、调节蛋白和底物相关(9)。也有证据表明有丝分裂过程中APC6和其他TPR亚基的磷酸化在调节TPR亚基和底物识别亚基如Cdc20的联合过程中，起到功能性的作用(10)。

1. Qiao, X. et al. (2010) Cell Cycle 9, 3904-12.
2. Harper, J.W. et al. (2002) Genes Dev 16, 2179-206.
3. Chang, L. et al. (2014) Nature 513, 388-93.
4. Carroll, C.W. and Morgan, D.O. (2002) Nat Cell Biol 4, 880-7.
5. Gmachl, M. et al. (2000) Proc Natl Acad Sci U S A 97, 8973-8.
6. Leverson, J.D. et al. (2000) Mol Biol Cell 11, 2315-25.
7. Kraft, C. et al. (2005) Mol Cell 18, 543-53.
8. Glotzer, M. et al. (1991) Nature 349, 132-8.
9. Pfleger, C.M. and Kirschner, M.W. (2000) Genes Dev 14, 655-65.
10. Schreiber, A. et al. (2011) Nature 470, 227-32.
11. Kraft, C. et al. (2003) EMBO J 22, 6598-609.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Bovine, Dog, Pig

Specificity / Sensitivity

APC6 (D8D8) Rabbit mAb recognizes endogenous levels of total APC6 protein. Based upon sequence alignment, this antibody is not predicted to cross-react with either APC8/CDC23 or APC3/CDC27.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human APC6 protein.

Background

Cell proliferation in all eukaryotic cells depends strictly upon the ubiquitin ligase (E3) activity of the anaphase promoting complex/cyclosome (APC/C), whose main function is to trigger the transition of the cell cycle from metaphase to anaphase. APC/C is a 1.5 MDa protein complex found in the nucleus of interphase cells. This complex diffuses throughout the cytoplasm and associates with parts of the spindle apparatus during mitosis. APC/C performs its various functions by promoting the assembly of polyubiquitin chains on substrate proteins, which targets these proteins for degradation by the 26S proteasome (1,2). In humans, twelve different APC/C subunits have been identified. Like all E3 enzymes, APC/C utilizes ubiquitin residues that have been activated by E1 enzymes and then transferred to E2 enzymes. Indeed APC/C has been shown to transiently interact with UBCH5 and UBCH10 E2 enzymes, in part, via the RING-finger domain-containing subunit, APC11 (3-5). In addition to E2 enzymes, APC/C activity is also strictly dependent upon one of several cofactors that associate with APC/C during specific phases of the cell cycle. The best studied of these are Cdc20 and Cdh1, which contain a C-terminal WD40 domain and participate in the recognition of APC/C substrates by interacting with specific recognition elements in these substrates (6), called D-Boxes (7) and KEN-boxes (8).APC6/CDC16 is a component of the tetratricopeptide repeat (TPR) sub-complex of the APC/C, which also consists of APC8/CDC23 and APC3/CDC27. It is thought that this sub-complex plays an important role in coordinating the juxtaposition of the catalytic and substrate recognition modules relative to co-activator, regulatory proteins, and substrates (9). There is also evidence suggesting that phosphorylation of APC6 and the other TPR subunits during mitosis plays a functional role in regulating the association between TPR subunits and substrate recognition subunits such as Cdc20 (10).

1. Qiao, X. et al. (2010) Cell Cycle 9, 3904-12.
2. Harper, J.W. et al. (2002) Genes Dev 16, 2179-206.
3. Chang, L. et al. (2014) Nature 513, 388-93.
4. Carroll, C.W. and Morgan, D.O. (2002) Nat Cell Biol 4, 880-7.
5. Gmachl, M. et al. (2000) Proc Natl Acad Sci U S A 97, 8973-8.
6. Leverson, J.D. et al. (2000) Mol Biol Cell 11, 2315-25.
7. Kraft, C. et al. (2005) Mol Cell 18, 543-53.
8. Glotzer, M. et al. (1991) Nature 349, 132-8.
9. Pfleger, C.M. and Kirschner, M.W. (2000) Genes Dev 14, 655-65.
10. Schreiber, A. et al. (2011) Nature 470, 227-32.
11. Kraft, C. et al. (2003) EMBO J 22, 6598-609.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.