Ki-67 (8D5) Mouse mAb

• 产品编号：CST-9449S      品牌：CST       原厂货号：9449S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Ki-67 (8D5) 小鼠mAb可以识别内源性总的Ki-67蛋白。IHC-P的物种交叉反应是任何小鼠，在IF-IC和F中是人，小鼠和大鼠。单克隆抗体由合成肽段免疫动物产生，该肽段与人Ki-67蛋白的氨基端对应。.Ki-67，以其发现地命名（Kiel大学，德国），作为一个细胞核非组蛋白（1）通常在增殖的细胞中表达并且在休眠细胞中不表达（2）。Ki-67会让增殖细胞停滞在G1，S，G2和有丝分裂期，但是不会停滞在G0期。研究显示高表达的Ki-67与低乳腺癌存活率相关（3）。研究已经将Ki-67与其他的标记，作为乳腺癌和其他恶性疾病中的潜在的预后和预测标记（4）。

1. Gerdes, J. et al. (1983) Int J Cancer 31, 13-20.
2. Weigel, M.T. and Dowsett, M. (2010) Endocr Relat Cancer 17, R245-62.
3. Jones, R.L. et al. (2009) Breast Cancer Res Treat 116, 53-68.
4. Yerushalmi, R. et al. (2010) Lancet Oncol 11, 174-83.

原厂资料：

Specificity / Sensitivity

Ki-67 (8D5) Mouse mAb recognizes endogenous levels of total Ki-67 protein. Species cross-reactivity for IHC-P is human and mouse, for IF-IC and F, it is human, mouse and rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Ki-67 protein. 

Background

Ki-67, named after the location where it was discovered (Kiel University, Germany), is a nuclear nonhistone protein (1) that is universally expressed among proliferating cells and absent in quiescent cells (2). Ki-67 detects proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase. Research studies have shown that high levels of Ki-67 are associated with poorer breast cancer survival (3). Research studies have explored the use of Ki-67, along with other markers, as potential prognostic or predictive markers in breast cancer and other malignant diseases (4).

1. Gerdes, J. et al. (1983) Int J Cancer 31, 13-20.
2. Weigel, M.T. and Dowsett, M. (2010) Endocr Relat Cancer 17, R245-62.
3. Jones, R.L. et al. (2009) Breast Cancer Res Treat 116, 53-68.
4. Yerushalmi, R. et al. (2010) Lancet Oncol 11, 174-83.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.