GST (26H1) Mouse mAb (Alexa Fluor? 594 Conjugate)

• 产品编号：CST-9440S      品牌：CST       原厂货号：9440S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

GST (26H1) Mouse mAb (Alexa Fluor® 594 Conjugate)能检测转染的谷胱甘肽S-转移酶（GST）融合蛋白。单克隆抗体通过用GST融合蛋白免疫动物制备。本抗体与Alexa Fluor® 594荧光染料结合，并且在人类细胞中进行了免疫荧光分析的内部测试。本抗体预计有与unconjugated GST (26H1) Mouse mAb #2624一样的种交叉反应。附加表位对于用免疫印迹、免疫沉淀和免疫染色技术来进行蛋白质的标记和检测很有用。因为它们很小，不大可能会影响被标记蛋白的生化特性。谷胱甘肽S-转移酶(GST)是一种广泛使用的融合伴侣，它能提供一个容易检测到的标签和一个简易的纯化过程，纯化后对相关蛋白的生物学功能基本没有影响。在过去十年中，出现了许多带有GST标签的原核或真核表达载体。

1. Guan, K.L. and Dixon, J.E. (1991) Anal Biochem 192, 262-7.
2. Davies, A.H. et al. (1993) Biotechnology (N Y) 11, 933-6.
3. Yu, J. et al. (1998) Mol Cell Biol 18, 1379-87.

原厂资料：

Specificity / Sensitivity

GST (26H1) Mouse mAb (Alexa Fluor® 594 Conjugate) detects transfected glutathione S-transferase (GST) fusion proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a GST fusion protein.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 594 fluorescent dye and tested in-house for immunofluorescent analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated GST (26H1) Mouse mAb #2624.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (1-3).

1. Guan, K.L. and Dixon, J.E. (1991) Anal Biochem 192, 262-7.
2. Davies, A.H. et al. (1993) Biotechnology (N Y) 11, 933-6.
3. Yu, J. et al. (1998) Mol Cell Biol 18, 1379-87.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4ºC. Protect from light. Do not
freeze.