N-Myc Antibody

• 产品编号：CST-9405S      品牌：CST       原厂货号：9405S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

该抗体能够检测内源的人N-Myc，及转染的小鼠N-Myc，其与其他Myc家族成员不发生交叉反应。该多克隆抗体是采用合成的人N-Myc 351位赖氨酸周围氨基序列相应的肽段免疫动物而生产制备，通过蛋白A和肽亲和色普法纯化。Myc/Max/Mad家族成员网络作为转录因子，而发挥多方面调节细胞行为的作用，调节细胞增殖、分化和凋亡 (1)。这些蛋白共同含有一个基本螺旋-环-螺旋亮氨酸拉链(bHLH-ZIP)基序，这是二聚化和与DNA结合所必需。最早发现 Max 具有与c-Myc 相结合的能力，是Myc结合DNA启动转录所必须的因子（2）。之后，Max被视为转录网络的一个核心成分，它与Myc 和Mad家族其它成员和形成同源或异源二聚体（1）。Max与Myc 或 Mad的联合作用对转录调节和细胞行为有着相反的作用（1）。Mad家族成员由四种相关蛋白组成，Mad1、Mad2 (Mxi1)、Mad3 和 Mad4，和相关性更小一些的bHLH-ZIP家族成员Mnt 和 Mga。与Myc一样，Mad蛋白通过短半衰期来调节。通常，Mad 蛋白家族成员干扰Myc介导的过程，例如增殖、转化和通过抑制转录而抑制的凋亡（3，4）。人类Myc家族包括5个基因：c-Myc、N-Myc、L-Myc、R-Myc和B-Myc。c-Myc在很多增殖细胞中表达，N-Myc的表达非常受限，在胚胎发育过程中以及成体B细胞的发育中高表达。这些表达模式和N-Myc靶向性缺失结果表明，N-Myc在组织发育和分化中发挥重要作用(5)。另外，研究表明，人类成神经细胞瘤中N-Myc的扩增或过表达与其迅速连续增殖和不良预后相关联（6,7）。

1. Baudino, T.A. and Cleveland, J.L. (2001) Mol Cell Biol 21, 691-702.
2. Blackwood, E.M. and Eisenman, R.N. (1991) Science 251, 1211-7.
3. Henriksson, M. and Lüscher, B. (1996) Adv Cancer Res 68, 109-82.
4. Grandori, C. et al. (2000) Annu Rev Cell Dev Biol 16, 653-99.
5. Sawai, S. et al. (1993) Development 117, 1445-1455.
6. Schwab, M. et al. (1984) Proc. Natl. Acad. Sci. USA 81, 4940-4944.
7. Brodeur, G.M. et al. (1984) Science 224, 1121-1124.

原厂资料：

Specificity / Sensitivity

N-Myc Antibody detects endogenous levels human N-Myc and transfected levels of mouse N-Myc. It does not cross-react with other Myc family members.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding lysine 351 of human N-Myc. Antibodies were purified by protein A and peptide affinity chromatography.

Background

Members of the Myc/Max/Mad network function as transcriptional regulators with roles in various aspects of cell behavior including proliferation, differentiation and apoptosis (1). These proteins share a common basic-helix-loop-helix leucine zipper (bHLH-ZIP) motif required for dimerization and DNA-binding. Max was originally discovered based on its ability to associate with c-Myc and found to be required for the ability of Myc to bind DNA and activate transcription (2). Subsequently, Max has been viewed as a central component of the transcriptional network, forming homodimers as well as heterodimers with other members of the Myc and Mad families (1). The association between Max and either Myc or Mad can have opposing effects on transcriptional regulation and cell behavior (1). The Mad family consists of four related proteins; Mad1, Mad2 (Mxi1), Mad3 and Mad4, and the more distantly related members of the bHLH-ZIP family, Mnt and Mga. Like Myc, the Mad proteins are tightly regulated with short half-lives. In general, Mad family members interfere with Myc-mediated processes such as proliferation, transformation and prevention of apoptosis by inhibiting transcription (3,4).In humans the Myc family consists of 5 genes: c-Myc, N-Myc, L-Myc, R-Myc, and B-Myc. While c-Myc is expressed in many proliferating cells, N-Myc expression is very restricted, with highest levels in during embryonic development and then in the adult during B-cell development. These expression patterns and results from targeted deletion of N-Myc suggest that N-Myc plays an important role in tissue development and differentiation (5). In addition, amplification or overexpression of N-Myc has been found in human neuroblastomas and is associated with rapid progression and poor prognosis (6,7).

1. Baudino, T.A. and Cleveland, J.L. (2001) Mol Cell Biol 21, 691-702.
2. Blackwood, E.M. and Eisenman, R.N. (1991) Science 251, 1211-7.
3. Henriksson, M. and Lüscher, B. (1996) Adv Cancer Res 68, 109-82.
4. Grandori, C. et al. (2000) Annu Rev Cell Dev Biol 16, 653-99.
5. Sawai, S. et al. (1993) Development 117, 1445-1455.
6. Schwab, M. et al. (1984) Proc. Natl. Acad. Sci. USA 81, 4940-4944.
7. Brodeur, G.M. et al. (1984) Science 224, 1121-1124.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibody