c-Myc Antibody

• 产品编号：CST-9402S      品牌：CST       原厂货号：9402S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

c-Myc抗体能够检测内源c-Myc总蛋白。不推荐该蛋白用于检测Myc-标签融合蛋白（推荐使用CST的#2276 或 #2278）。c-Myc抗体能够检测内源c-Myc总蛋白。不推荐该蛋白用于检测Myc-标签融合蛋白（推荐使用CST的#2276 或 #2278）。Myc/Max/Mad网络成员作为转录调控因子,在细胞增殖、分化和凋亡等多种细胞行为方面发挥着重要功能（1）。这些蛋白质共享一个共同的基础螺旋-环-螺旋亮氨酸拉链(bHLH-ZIP)基序，这是二聚化和DNA结合所需的。Max最初是因其与c-Myc的互作能力而被发现，发现它对于Myc蛋白与DNA结合和激活转录过程是必须的（2）。随后，Max一直被视为转录网络的核心组成部分，与Myc和Mad家族其他成员形成同源二聚体和异源二聚体（1）。Max与Myc或Mad的互作对于转录调控和细胞行为可以有相反的作用（1）。Mad家族包括四个相关的蛋白质，Mad1、Mad2 (Mxi1)、Mad3和Mad4，以及bHLH-ZIP 家族最不相关的成员Mnt和Mga。与Myc一样，Mad蛋白严格被短半衰期调控。在一般情况下，Mad家族成员通过抑制转录干扰Myc蛋白介导的生命过程，如增殖、细胞凋亡的转化和阻止（3,4）。

1. Baudino, T.A. and Cleveland, J.L. (2001) Mol Cell Biol 21, 691-702.
2. Blackwood, E.M. and Eisenman, R.N. (1991) Science 251, 1211-7.
3. Henriksson, M. and Lüscher, B. (1996) Adv Cancer Res 68, 109-82.
4. Grandori, C. et al. (2000) Annu Rev Cell Dev Biol 16, 653-99.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Pig

Specificity / Sensitivity

c-Myc Antibody detects endogenous levels of total c-Myc protein. This antibody is not recommended for detection of Myc-tagged fusion proteins (use Cell Signaling Technology cat. #2276 or #2278).

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues of c-Myc. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Members of the Myc/Max/Mad network function as transcriptional regulators with roles in various aspects of cell behavior including proliferation, differentiation and apoptosis (1). These proteins share a common basic-helix-loop-helix leucine zipper (bHLH-ZIP) motif required for dimerization and DNA-binding. Max was originally discovered based on its ability to associate with c-Myc and found to be required for the ability of Myc to bind DNA and activate transcription (2). Subsequently, Max has been viewed as a central component of the transcriptional network, forming homodimers as well as heterodimers with other members of the Myc and Mad families (1). The association between Max and either Myc or Mad can have opposing effects on transcriptional regulation and cell behavior (1). The Mad family consists of four related proteins; Mad1, Mad2 (Mxi1), Mad3 and Mad4, and the more distantly related members of the bHLH-ZIP family, Mnt and Mga. Like Myc, the Mad proteins are tightly regulated with short half-lives. In general, Mad family members interfere with Myc-mediated processes such as proliferation, transformation and prevention of apoptosis by inhibiting transcription (3,4).

1. Baudino, T.A. and Cleveland, J.L. (2001) Mol Cell Biol 21, 691-702.
2. Blackwood, E.M. and Eisenman, R.N. (1991) Science 251, 1211-7.
3. Henriksson, M. and Lüscher, B. (1996) Adv Cancer Res 68, 109-82.
4. Grandori, C. et al. (2000) Annu Rev Cell Dev Biol 16, 653-99.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody