Phospho-CRMP-2 (Thr514) Antibody

• 产品编号：CST-9397S      品牌：CST       原厂货号：9397S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-CRMP-2 (Thr514)Antibody 兔多抗识别内源性的Thr514磷酸化的CRMP-2蛋白。该多克隆抗体由合成的人类CRMP-2蛋白Thr514附近肽段免疫动物而制成。该抗体使用多肽亲和层析纯化而得。Collapsin反应调节蛋白-2（CRMP-2）在神经系统的发育过程中高水平表达，在轴突生长中起着至关重要的作用，它决定轴突/树突的命运和建立神经元极性（1,2）。CRMP-2结合微管蛋白二聚体，促进微管组装，从而促进轴突伸长和分枝（3）。GSK-3β磷酸化CRMP-2的Thr514，使其失活。CRMP-2被CDK5在Ser522磷酸化，被GSK-3β在Thr518磷酸化（2）。CRMP-2的磷酸化，会降低其与微管蛋白结合的能力，可由NT-3和BDNF通过PI3激酶/ Akt的途径抑制（2）。CRMP-2也介导semaphorin诱导的生长锥崩溃（4）。CRMP-2的高磷酸化在阿尔茨海默病斑块中被发现，同时伴有升高的GSK-3β活性（5）。

1. Gu, Y. and Ihara, Y. (2000) J Biol Chem 275, 17917-20.
2. Yoshimura, T. et al. (2005) Cell 120, 137-49.
3. Fukata, Y. et al. (2002) Nat Cell Biol 4, 583-91.
4. Goshima, Y. et al. (1995) Nature 376, 509-14.
5. Cole, A.R. et al. (2004) J Biol Chem 279, 50176-80.

原厂资料：

Specificity / Sensitivity

Phospho-CRMP-2 (Thr514) Antibody detects endogenous levels of CRMP-2 only when phosphorylated at Thr514.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Thr514 of human CRMP-2. Antibodies are purified by peptide affinity chromatography.

Background

Collapsin Response Mediator Protein-2 (CRMP-2) is expressed at high levels in the developing nervous system and plays a critical role in axonal outgrowth by specifying axon/dendrite fate and establishing neuronal polarity (1,2). CRMP-2 enhances axon elongation and branching by binding to tubulin heterodimers to promote microtubule assembly (3). GSK-3β inactivates CRMP-2 by phosphorylating it at Thr514. CRMP-2 is primed following phosphorylation at Ser522 by CDK5 and at Thr518 by GSK-3β (2). Phosphorylation of CRMP-2, which decreases tubulin binding ability, can be inhibited by NT-3 and BDNF through the PI3 kinase/Akt pathway (2). CRMP-2 also mediates semaphorin-induced growth cone collapse (4). Hyperphosphorylation of CRMP-2 and is found in Alzheimer disease plaques with concurrent elevated GSK-3β activity in these patients (5).

1. Gu, Y. and Ihara, Y. (2000) J Biol Chem 275, 17917-20.
2. Yoshimura, T. et al. (2005) Cell 120, 137-49.
3. Fukata, Y. et al. (2002) Nat Cell Biol 4, 583-91.
4. Goshima, Y. et al. (1995) Nature 376, 509-14.
5. Cole, A.R. et al. (2004) J Biol Chem 279, 50176-80.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.