Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101)

• 产品编号：CST-9391S      品牌：CST       原厂货号：9391S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101)识别脯氨酸之前的磷酸化苏氨酸。它与磷酸化苏氨酸-脯氨酸基序发生反应，且不与背景氨基酸序列有关。此抗体是磷酸化特异性的，但是不能识别缺乏跟随脯氨酸的磷酸化苏氨酸。此抗体与磷酸化酪氨酸没有交叉反应，但与一些包含磷酸化丝氨酸-脯氨酸模体的磷酸化丝氨酸蛋白（如磷酸化Elk-1）有交叉反应。（美国专利号：6,441,140、6,982,318、7,259,022、7,344,714；U.S.S.N. 11,484,485；及所有国外相应专利）。该单克隆抗体用合成的含磷酸化苏氨酸-脯氨酸的肽段免疫动物制备。该抗体是鼠IgM克隆，可被抗鼠Ig（全分子）二抗识别。MAPK和CDK丝氨酸苏氨酸蛋白激酶家族在细胞信号和周期调控中扮演重要角色。这些激酶磷酸化脯氨酸之前的苏氨酸或丝氨酸（1-6）。为了确认新的MAPK和CDK底物，Cell Signaling Technology公司开发了识别有脯氨酸之前的磷酸化苏氨酸的抗体。使用大量磷酸化苏氨酸-脯氨酸肽段进行的ELISA显示，Phospho-Threonine-Proline Monoclonal Antibody (P-Thr-Pro-101)识别磷酸化苏氨酸-脯氨酸模体，而不考虑其背景序列。对Jurkat细胞抽提液进行的二维凝胶Wester分析显示它也能与很多包含磷酸化苏氨酸-脯氨酸的蛋白质发生交叉反应。

1. Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.
2. Seger, R. and Krebs, E.G. (1995) FASEB J 9, 726-35.
3. Nurse, P. (2000) Cell 100, 71-8.
4. Cross, T.G. et al. (2000) Exp Cell Res 256, 34-41.
5. Yang, C.C. et al. (1998) J Protein Chem 17, 329-35.
6. Reynolds, C.H. et al. (2000) J Neurochem 74, 1587-95.

原厂资料：

Specificity / Sensitivity

Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101) detects phospho-threonine only when followed by proline. It reacts with proteins and peptides phosphorylated at the Thr-Pro motif in an otherwise highly context-independent fashion. The antibody is phospho-specific, but does not recognize phospho-threonine in the absence of an adjacent proline. The antibody does not react with phospho-tyrosine but does react with some phospho-serine peptides containing the phospho-serine-proline motif (e.g., phospho-Elk-1). (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic phospho-threonine-proline-containing peptides . This antibody is a mouse IgM clone and can be recognized by anti-mouse Ig (whole molecule) secondary antibody.

Background

The MAPK and CDK families of serine/threonine protein kinases play important roles in cell signaling and cell cycle control. These kinases phosphorylate threonine or serine followed by a proline residue (1-6). To study and discover new MAPK and CDK substrates, Cell Signaling Technology has developed antibodies that bind to phospho-threonine followed by proline.As determined by ELISA using a wide variety of phospho-Thr-Pro peptides, Phospho-Threonine-Proline Monoclonal Antibody (P-Thr-Pro-101) recognizes the phospho-Thr-Pro motif in a highly context-independent fashion. It also interacts with a broad range of phospho-Thr-Pro-containing proteins as determined by western analysis of nocodazole-treated Jurkat cell extracts resolved on 2-D gels.

1. Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.
2. Seger, R. and Krebs, E.G. (1995) FASEB J 9, 726-35.
3. Nurse, P. (2000) Cell 100, 71-8.
4. Cross, T.G. et al. (2000) Exp Cell Res 256, 34-41.
5. Yang, C.C. et al. (1998) J Protein Chem 17, 329-35.
6. Reynolds, C.H. et al. (2000) J Neurochem 74, 1587-95.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.