Cytokeratin Antibody Sampler Kit

• 产品编号：CST-9384S      品牌：Cell Signaling Technology       原厂货号：9384S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

Pan-keratin (C11) mouse mAb识别内源性的keratin 4、5、6、8、10、13和18总蛋白，但是不能与其它keratin蛋白发生交叉反应。该试剂盒包含的其他每个抗体都识别各自特异的内源性keratin蛋白，但是不与其它keratin蛋白发生交叉反应。通过来自A431细胞的一个细胞骨架的制备去免疫动物从而生成出Pan-Keratin Mouse mAb (C11)。通过来自HeLa细胞的一个细胞骨架的制备去免疫动物从而生成出小鼠单抗。通过来自人源PMC-42乳腺癌细胞去免疫动物从而制备出Keratin 18 (DC10) Mouse mAb。通过人源乳腺上皮器官的去垢剂不溶物去免疫动物从而制备出Keratin 19 (BA17) Mouse mAb。通过人工合成人源keratin 7蛋白氨基末端相关的多肽片段去免疫动物从而制备出Keratin 7 抗体。通过人工合成人源keratin 7蛋白羧基末端相关的多肽片段去免疫动物从而制备出Keratin 17 (D73C7) XP^® 单克隆抗体。通过蛋白A和多肽亲和层析纯化多克隆抗体。Cytokeratin Antibody Sampler Kit提供一个经济手段去鉴定keratin蛋白存在的状态和蛋白水平。该试剂盒提供了足够进行四次Western Blot所需的一抗和二抗。角蛋白（细胞角蛋白）是中间丝蛋白主要在上皮细胞中表达。角蛋白的异二聚体由一个酸性角蛋白（或type I角蛋白，角蛋白9-23或）和一个碱性角蛋白（type II角蛋白，角蛋白1-8）聚合形成中间丝（1.2）。角蛋白的亚型在组织-和分化的特异性表达，这使他们可做为有用的生物标志（1）。研究显示角蛋白基因突变与肌肤失调，肝脏和胰腺疾病，以及肠道炎性疾病相关（3-6）。

1. Moll, R. et al. (1982) Cell 31, 11-24.
2. Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
3. Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4.
4. Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66.
5. Zatloukal, K. et al. (2004) J Pathol 204, 367-76.
6. Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85.

原厂资料：

Specificity / Sensitivity

The pan-keratin (C11) mouse mAb detects endogenous levels of total keratins 4, 5, 6, 8, 10, 13 and 18. The antibody does not cross-react with other keratins. Each of the remaining antibodies included in this kit detect endogenous levels of the specified keratin protein and do not cross-react with other keratin proteins.

Source / Purification

Pan-Keratin Mouse mAb (C11) is produced by immunizing animals with a cytoskeleton preparation from A431 cells. Keratin 8/18 (C51) Mouse mAb is produced by immunizing animals with a cytoskeleton preparation from HeLa cells. Keratin 18 (DC10) Mouse mAb is produced by immunizing animals with human PMC-42 breast carcinoma cells. Keratin 19 (BA17) Mouse mAb is produced by immunizing animals with detergent-insoluble extract of human mammary epithelial organoids. Keratin 7 Antibody is generated by immunizing animals with a synthetic peptide corresponding to amino acids at the amino terminus of human keratin 7. Keratin 17 (D73C7) XP® Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acids near the carboxy terminus of human keratin 17. Polyclonal antibodies are purified by Protein A and peptide affinity chromatography.

Description

The Cytokeratin Antibody Sampler Kit provides an economical means to evaluate the presence and status of selected keratin proteins. The kit provides enough primary and secondary antibodies to perform four Western blot experiments per primary antibody.

Background

Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as biomarkers (1). Mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).

1. Moll, R. et al. (1982) Cell 31, 11-24.
2. Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
3. Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4.
4. Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66.
5. Zatloukal, K. et al. (2004) J Pathol 204, 367-76.
6. Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibodies