TCF/LEF1 Antibody Sampler Kit

产品编号：CST-9383S 品牌：Cell Signaling Technology 原厂货号：9383S
产品分类：抗体 > 一抗 > 复合抗体试剂盒
应用分类：

包装：

1 Kit

运保温度:

–20°C

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其它组分：

TCF1 (C63D9) Rabbit mAb 【子货号：#2203，包装：40 µl，，运保温度：–20°C】

LEF1 (C12A5) Rabbit mAb 【子货号：#2230，包装：40 µl，，运保温度：–20°C】

TCF4 (C48H11) Rabbit mAb 【子货号：#2569，包装：40 µl，，运保温度：–20°C】

TCF3 (D15G11) Rabbit mAb 【子货号：#2883，包装：40 µl，，运保温度：–20°C】

Anti-rabbit IgG, HRP-linked Antibody 【子货号：#7074，包装：100 µl，，运保温度：–20°C】

描述：

本试剂盒中每个抗体均可以特异识别对应的内源性总蛋白并与其他家族成员没有交叉反应。TCF1 (C63D9) Rabbit mAb不识别缺乏氨基端β-catenin结合结构域的显性负亚型TCF1。LEF1 (C12A5) Rabbit mAb不识别由替代启动子产生的显性负亚型TCF1。分别合成与人TCF1蛋白 95位脯氨酸，TCF396位谷氨酰胺，330亮氨酸和LEF1 82位脯氨酸及其邻近氨基酸残基序列一致的多肽，多肽免疫动物获得单克隆抗体。TCF/LEF1 Antibody Sampler 试剂盒提供了经济合算的方式检测TCF/LEF1家族成员总蛋白。本试剂盒包含有足够4次western实验的一抗和二抗。LEF1和TCF是高迁移率族蛋白(HMG)中DNA结合蛋白家族的成员中的一类转录因子，包括：Lymphoid Enhancer Factor 1 (LEF1), T Cell Factor 1 (TCF1), TCF3, and TCF4 (1)。LEF1和TCF1最初被认为是调节早期淋巴发育的重要因子(2)，并作为Wnt的下游信号发挥作用。LEF1和TCF结合Wnt反应元件为β-catenin提供停泊位点，β-catenin随后转移到细胞核促进Wnt信号通路靶基因的转录激活(3)。在包括结肠癌（4，5）在内的多种癌症发生相关的Wnt信号通路的发展和异常活化中，都会伴随着LEF1和TCF表达水平的动态变化。

原厂资料：

Specificity / Sensitivity

Each antibody in this kit recognizes endogenous levels of total protein for the specified target and does not cross-react with other family members. TCF1 (C63D9) Rabbit mAb does not recognize the dominant negative isoforms of TCF1 lacking the amino-terminal β-catenin binding domain. LEF1 (C12A5) Rabbit mAb does not recognize the dominant negative forms of LEF1 generated by an alternative promoter.

Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic peptides corresponding to regions surrounding Pro95 of human TCF1 protein, Gln96 of human TCF3, Leu330 of human TCF4, and Pro82 of human LEF1.

Description

The TCF/LEF1 Antibody Sampler Kit provides an economical means of detecting total protein from the TCF/LEF1 family members. The kit contains enough primary and secondary antibodies to perform four Western blots with each antibody.

Background

LEF1 and TCF are members of the high mobility group (HMG) DNA binding protein family of transcription factors that consists of the following: Lymphoid Enhancer Factor 1 (LEF1), T Cell Factor 1 (TCF1), TCF3, and TCF4 (1). LEF1 and TCF1 were originally identified as important factors regulating early lymphoid development (2) and act downstream in Wnt signaling. LEF1 and TCF bind to Wnt response elements to provide docking sites for β-catenin, which translocates to the nucleus to promote the transcription of target genes upon activation of Wnt signaling (3). LEF1 and TCF are dynamically expressed during development and aberrant activation of the Wnt signaling pathway is involved in many types of cancers including colon cancer (4,5).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibodies.