Perilipin (D1D8) XP? Rabbit mAb

• 产品编号：CST-9349S      品牌：CST       原厂货号：9349S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Perilipin (D1D8) XP® Rabbit mAb兔单抗用于检测内源性perilipin总蛋白水平。单抗通过用与人perifilin蛋白Ile419位点处相同序列的合成肽免疫动物而制备获得甘油三酯作为人体首要的能量储备，主要以脂肪滴的形式存储于体内。Perilipin位于脂肪滴的外层，起着保护脂质，防止被脂酶裂解的作用(1-3)。许多证据 表明，PKA通过磷酸化perifipin来调控脂解作用(1,2,4,5)。perifipin的磷酸化使脂肪滴的形态发生改变，将脂肪滴暴露于内源性的脂酶中(如激素敏感型的 脂酶)(2)。因此，perilipin在脂质的储存中起着重要的作用(2,5)。

1. Greenberg, A.S. et al. (1991) J Biol Chem 266, 11341-6.
2. Brasaemle, D.L. (2007) J Lipid Res 48, 2547-59.
3. Ducharme, N.A. and Bickel, P.E. (2008) Endocrinology 149, 942-9.
4. Egan, J.J. et al. (1990) J Biol Chem 265, 18769-75.
5. Brasaemle, D.L. et al. (2009) Mol Cell Biochem 326, 15-21.

原厂资料：

Specificity / Sensitivity

Perilipin (D1D8) XP® Rabbit mAb detects endogenous levels of total perilipin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues around Ile419 of human perilipin protein.

Background

Triacylglycerol is stored in lipid droplets as a primary energy reserve. Perilipin is localized at the periphery of lipid droplets and serves as a protective coating against lipases (1-3). Evidence suggests that PKA regulates lipolysis by phosphorylating perilipin (1,2,4,5). Phosphorylation of perilipin results in the conformational change that exposes lipid droplets to endogenous lipases, such as hormone-sensitive lipases (2). Hence, perilipin plays a pivotal role in lipid storage (2,5).

1. Greenberg, A.S. et al. (1991) J Biol Chem 266, 11341-6.
2. Brasaemle, D.L. (2007) J Lipid Res 48, 2547-59.
3. Ducharme, N.A. and Bickel, P.E. (2008) Endocrinology 149, 942-9.
4. Egan, J.J. et al. (1990) J Biol Chem 265, 18769-75.
5. Brasaemle, D.L. et al. (2009) Mol Cell Biochem 326, 15-21.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.