FAK Antibody Sampler Kit

• 产品编号：CST-9330S      品牌：Cell Signaling Technology       原厂货号：9330S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

FAK抗体样品试剂盒中的每个抗体检测内源性靶标蛋白的水平，不典型与其他蛋白交叉反应。 激活状态抗体仅仅在指定残基磷酸化时识别靶标蛋白， 磷酸化FAK (酪氨酸397)抗体肯那个与过表达酪氨酸磷酸化的EGF受体交叉反应。活化状态多克隆抗体由合成的磷酸化肽段免疫动物产生，该肽段对应人FAK蛋白的酪氨酸397, 酪氨酸576/577 和酪氨酸 925附近残基。FAK抗体由合成肽段免疫动物产生，该肽段对应人FAK蛋白710氨基酸附近残基。抗体由蛋白A和肽段亲和层析技术纯化。FAK抗体样品试剂盒提供经济手段评估总的FAK蛋白水平，以及FAK在特异位点的磷酸化，试剂盒含有足够第一和第二抗体，每个抗体能做四次western blots。黏着斑激酶(FAK)是广泛表达的细胞质蛋白酪氨酸激酶，涉及整合素调控的信号转导，它在一些生物过程包括细胞扩散、转移和存活的控制中起重要作用（1）。FAK被整合素聚集而激活，导致在397位酪氨酸的自磷酸化，这是一个Src家族激酶PI3K和PL Cγ的结合位点（2-5）。Src家族激酶的招募导致FAK催化结构域酪氨酸407, 酪氨酸576, and 酪氨酸577的磷酸化，以及FAK的C末端区域871位酪氨酸和925位酪氨酸磷酸化（6,7）。

1. Parsons, J.T. et al. (2000) Oncogene 19, 5606-13.
2. Schaller, M.D. et al. (1994) Mol Cell Biol 14, 1680-8.
3. Cobb, B.S. et al. (1994) Mol Cell Biol 14, 147-55.
4. Chen, H.C. et al. (1996) J Biol Chem 271, 26329-34.
5. Zhang, X. et al. (1999) Proc Natl Acad Sci U S A 96, 9021-6.
6. Calalb, M.B. et al. (1995) Mol Cell Biol 15, 954-63.
7. Schlaepfer, D.D. et al. (1994) Nature 372, 786-791.

原厂资料：

Specificity / Sensitivity

Each antibody in the FAK Antibody Sampler Kit detects endogenous levels of its target protein and does not typically cross react with other proteins. Activation state antibodies recognize target proteins only when phosphorylated at the indicated residue. Phospho-FAK (Tyr397) Antibody may cross-react with overexpressed tyrosine phosphorylated EGF receptors.

Source / Purification

Activation state polyclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Tyr397, Tyr576/577 and Tyr 925 of human FAK protein. FAK Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding amino acid 710 of human FAK protein. Antibodies are purified by protein A and peptide affinity chromatography.

Description

The FAK Antibody Sampler Kit provides an economical means of evaluating total FAK protein levels as well as FAK phosphorylated at specific sites. The kit contains enough primary and secondary antibody to perform four western blots with each antibody.

Background

Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. It plays an important role in the control of several biological processes, including cell spreading, migration, and survival (1). Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397, which is a binding site for the Src family kinases PI3K and PLCγ (2-5). Recruitment of Src family kinases results in the phosphorylation of Tyr407, Tyr576, and Tyr577 in the catalytic domain, and Tyr871 and Tyr925 in the carboxy-terminal region of FAK (6,7).

1. Parsons, J.T. et al. (2000) Oncogene 19, 5606-13.
2. Schaller, M.D. et al. (1994) Mol Cell Biol 14, 1680-8.
3. Cobb, B.S. et al. (1994) Mol Cell Biol 14, 147-55.
4. Chen, H.C. et al. (1996) J Biol Chem 271, 26329-34.
5. Zhang, X. et al. (1999) Proc Natl Acad Sci U S A 96, 9021-6.
6. Calalb, M.B. et al. (1995) Mol Cell Biol 15, 954-63.
7. Schlaepfer, D.D. et al. (1994) Nature 372, 786-791.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 μg/ml BSA, 50% glycerol. Store at –20°C.
Do not aliquot the antibodies