Phospho-Tyk2 (Tyr1054/1055) Antibody

• 产品编号：CST-9321S      品牌：CST       原厂货号：9321S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Tyk2 (Tyr1054/1055) Antibody能够检测内源在tyrosine1054/1055位点磷酸化的Tyk2蛋白，抗体不与其它位点磷酸化的Tyk2蛋白交叉反应。多克隆抗体是通过合成对应人源性Tyk2 Tyr1054/1055 位点周围的磷肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析法纯化。Tyk2是Jak酪氨酸蛋白激酶家族的一员，与很多细胞因子的受体有关并受到他们的激活，包括 IL-13, IL-6 家族, IL-10, 干扰素-alpha 和干扰素- beta (1-3)。 配体结合后，Tyk2 通过Tyr1054 和/或者 Tyr1055位点的磷酸化而激活 (4)。在IFN-beta信号级联反应中，Tyk2是Stat3的酪氨酸磷酸化所必需的(5)。

1. Velazquez, L. et al. (1995) J. Biol. Chem. 270, 3327-34.
2. Stahl, N. et al. (1994) Science 263, 92-5.
3. Leonard, W.J. (1998) Annu. Rev. Immunol. 16, 293-322.
4. Grauzzi, M.C. et al. (1996) J. Biol. Chem. 271, 20494-500.
5. Rani, M.R. et al. (1999) J. Biol. Chem. 274, 32507-11.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

Phospho-Tyk2 (Tyr1054/1055) Antibody detects endogenous levels of Tyk2 only when phosphorylated at tyrosine1054/1055. The antibody does not cross-react with Tyk2 phosphorylated at other sites.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1054/1055 of human Tyk2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Tyk2, a member of the Jak family of protein tyrosine kinases, associates with and is activated by receptors for many cytokines including IL-13, the IL-6 family, IL-10, and interferon-alpha and beta (1-3). Following ligand binding, Tyk2 is activated by phosphorylation on Tyr1054 and/or Tyr1055 (4). Tyk2 is required for the tyrosine phosphorylation of Stat3 in the IFN-beta signaling cascade (5).

1. Velazquez, L. et al. (1995) J. Biol. Chem. 270, 3327-34.
2. Stahl, N. et al. (1994) Science 263, 92-5.
3. Leonard, W.J. (1998) Annu. Rev. Immunol. 16, 293-322.
4. Grauzzi, M.C. et al. (1996) J. Biol. Chem. 271, 20494-500.
5. Rani, M.R. et al. (1999) J. Biol. Chem. 274, 32507-11.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody