Fascin (D1A8) Rabbit mAb

• 产品编号：CST-9269S      品牌：CST       原厂货号：9269S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Fascin (D1A8) Rabbit mAb可以识别内源性总的fascin蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人fascin蛋白的Arg201邻近残基序列一致。Fascin是一个单体，球形蛋白在调控皮层actin细胞骨架的结构和功能时发挥作用（1）。Fascin促进细胞突起形成时平行的肌动蛋白丝交联（板状伪足和丝状伪足），因此在调控细胞迁移的过程中发挥了关键作用（2）。已有报道fascin可能通过不依赖于actin-结合能力的机制调控了丝状伪足的形成（3），尽管该机制尚不可知。研究显示fascin表达升高与赘生性细胞的运动和侵润性增强有关，包括乳腺癌、结肠癌、前列腺癌和食管鳞状细胞癌（4-6）。Fascin可以在体内外都与β-catenin的armadillo-重复区域结合，它与β-catenin和钙黏连蛋白同时位于迁移细胞的前缘（7）。

1. Hoelzle, M.K. and Svitkina, T. (2012) Mol Biol Cell 23, 310-23.
2. Adams, J.C. (2004) Curr Opin Cell Biol 16, 590-6.
3. Zanet, J. et al. (2012) J Cell Biol 197, 477-86.
4. Hashimoto, Y. et al. (2005) Int J Biochem Cell Biol 37, 1787-804.
5. Stewart, C.J. et al. (2012) J Clin Pathol 65, 213-7.
6. Alam, H. et al. (2012) BMC Cancer 12, 32.
7. Tao, Y.S. et al. (1996) J Cell Biol 134, 1271-81.

原厂资料：

Specificity / Sensitivity

Fascin (D1A8) Rabbit mAb recognizes endogenous levels of total fascin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg201 of human fascin protein.

Background

Fascin is a monomeric, globular protein that plays a central role in regulating the structure and function of the cortical actin cytoskeleton (1). Fascin promotes cross-linkage of parallel actin filaments during the formation of cell protrusions (lamellipodia and filopodia), and therefore plays an important role in regulating cell migration (2). It has been reported that fascin may also regulate filopodia formation by a mechanism independent of its actin-bundling functions (3), though less is known about this mechanism of action. Research studies have shown that increased fascin expression is associated with increased motility and invasiveness of neoplastic cells, including breast, colon, prostate, and esophageal squamous cell carcinomas (4-6). Fascin binds to the armadillo-repeat domain of β-catenin in vitro and in vivo, and has been shown to co-localize with β-catenin and cadherins at the leading edge of migratory cells (7).

1. Hoelzle, M.K. and Svitkina, T. (2012) Mol Biol Cell 23, 310-23.
2. Adams, J.C. (2004) Curr Opin Cell Biol 16, 590-6.
3. Zanet, J. et al. (2012) J Cell Biol 197, 477-86.
4. Hashimoto, Y. et al. (2005) Int J Biochem Cell Biol 37, 1787-804.
5. Stewart, C.J. et al. (2012) J Clin Pathol 65, 213-7.
6. Alam, H. et al. (2012) BMC Cancer 12, 32.
7. Tao, Y.S. et al. (1996) J Cell Biol 134, 1271-81.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody