GSK-3 Fusion Protein

• 产品编号：CST-9237S      品牌：CST       原厂货号：9237S
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 其它纯化的蛋白及酶
• 应用分类：

描述：

GSK-3 融合蛋白可以作为Akt激酶活性检测分析中的底物。它与GSK-3 α/β crosstide一起融合表达，具体对应于GSK-3 α/β的Ser21/9位点周围序列(CGPKGP-GRRGRRRTSSFAEG)。糖原合酶激酶-3（GSK-3）最初被确认为是一种能在胰岛素的刺激下参与调节糖原合成的酶（1）。GSK-3是一个广泛表达的丝氨酸/苏氨酸蛋白激酶，可以磷酸化并抑制糖原合成酶的活性。GSK-3是PI3K/ Akt细胞存活通路上的一个关键下游分子，其活性可以被Akt介导的GSK-3α的Ser21位点和GSK-3β的Ser9位点的磷酸化抑制（2,3）。GSK-3不仅参与盘基网柄菌细胞命运的调控，而且是果蝇，爪蟾和哺乳动物发育过程中所需的Wnt信号通路的一个组成部分（4）。GSK-3还参与调节Cyclin D1 蛋白降解和亚细胞定位（5）。

1. Welsh, G.I. et al. (1996) Trends Cell Biol 6, 274-9.
2. Srivastava, A.K. and Pandey, S.K. (1998) Mol Cell Biochem 182, 135-41.
3. Cross, D.A. et al. (1995) Nature 378, 785-9.
4. Nusse, R. (1997) Cell 89, 321-3.
5. Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.

原厂资料：

Description

GSK-3 Fusion Protein serves as a useful substrate for assaying Akt kinase activity. It is expressed as a protein fusion to the GSK-3 α/β crosstide, corresponding to residues surrounding GSK-3 α/β (Ser21/9) (CGPKGPGRRGRRRTSSFAEG).

Background

Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).

1. Welsh, G.I. et al. (1996) Trends Cell Biol 6, 274-9.
2. Srivastava, A.K. and Pandey, S.K. (1998) Mol Cell Biochem 182, 135-41.
3. Cross, D.A. et al. (1995) Nature 378, 785-9.
4. Nusse, R. (1997) Cell 89, 321-3.
5. Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.

注意事项：

Storage: Supplied in 20 mM Tris-HCl (pH 7.5 at 25°C), 50 mM
NaCl, 2 mM Na2EDTA, 1 mM dithiothreitol (DTT) and 50%
glycerol. Store at –20°C.