Phospho-MKK3 (Ser189)/MKK6 (Ser207) Antibody

• 产品编号：CST-9231S      品牌：CST       原厂货号：9231S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-MKK3/MKK6 (Ser189/207) Antibody兔多抗能检测内源性Ser189/207位点磷酸化的MKK3/MKK6蛋白水平。该抗体不能识别MEK1, MEK2和MKK4/SEK1蛋白的相应磷酸化残基。该多克隆抗体是采用合成的与人源MKK3蛋白Ser189/207位点周围残基相一致的磷酸化肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。MKK3和MKK6是两种密切相关的双特异性蛋白激酶，能够激活p38 MAPK（1-5）。MKK3和MKK6能够通过磷酸化p38 MAPK的活化位点Thr-Gly-Tyr从而使p38 MAPK激活，但不能磷酸化或激活Erk1/2或SAPK/JNK。p38 MAPK的磷酸化能够显著提高其磷酸化蛋白底物的能力，这些底物包括ATF-2和Elk-1。MKK3和MKK6能被不同形式的细胞压力和炎症细胞因子激活（4,5）。通过磷酸化MKK3的Ser189和Thr193位点(2)以及MKK6的Ser207和Thr211位点（4,5）可以激活MKK3和MKK6。

1. Derijard, B. et al. (1995) Science 267, 682-685.
2. Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
3. Sluss, H.K. et al. (1994) Mol. Cell. Biol. 14, 8376-8384.
4. Raingeaud, J. et al. (1996) Mol. Cell. Biol. 16(3), 1247-1255.
5. Han, J. et al. (1996) J. Biol. Chem. 271, 2886-2891.

原厂资料：

Specificity / Sensitivity

Phospho-MKK3/MKK6 (Ser189/207) Antibody detects endogenous levels of MKK3/MKK6 only when phosphorylated at Ser189/207. The antibody does not recognize the corresponding phosphorylated residues of MEK1, MEK2 or MKK4/SEK1.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser189/207 of human MKK3. Antibodies are purified by protein A and peptide affinity chromatography.

Background

MKK3 and MKK6 are two closely related dual-specificity protein kinases that activate p38 MAP kinase (1-5). MKK3 and MKK6 both phosphorylate and activate p38 MAP kinase at its activation site Thr-Gly-Tyr but do not phosphorylate or activate Erk1/2 or SAPK/JNK. Phosphorylation of p38 MAP kinase dramatically stimulates its ability to phosphorylate protein substrates such as ATF-2 and Elk-1. MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines (4,5). Activation of MKK3 and MKK6 occurs through phosphorylation of serine and threonine residues at sites Ser189 and Thr193 for MKK3 (2) and Ser207 and Thr211 for MKK6 (4,5).

1. Derijard, B. et al. (1995) Science 267, 682-685.
2. Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
3. Sluss, H.K. et al. (1994) Mol. Cell. Biol. 14, 8376-8384.
4. Raingeaud, J. et al. (1996) Mol. Cell. Biol. 16(3), 1247-1255.
5. Han, J. et al. (1996) J. Biol. Chem. 271, 2886-2891.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.