FAAH1 Antibody (Rat Specific)

• 产品编号：CST-9179S      品牌：CST       原厂货号：9179S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

FAAH1 Antibody (Rat Specific)用于检测内源性FAAH1总蛋白水平。该多抗通过用与鼠FAAH1蛋白N端序列相同的合成肽免疫动物制备而来。 抗体的纯化采用蛋白酶A与肽段亲和层析相结合的方法。内源性大麻素在成瘾行为如毒品成瘾中起着作用(1)。脂肪酸酰胺水解酶1(FAAH1)是一种膜结合水解酶，负责将油酸酰胺转化成油酸(2)。该酶同样也可以将大麻素(作为内源性CB1大麻素受体的配体)转变成大麻酸，这提示它可以在脂肪酸酰胺的失活中发挥着某些作用(2)。FAAH1缺失小鼠的大脑中大麻素的水平显著上升，并且表现出对疼痛的不敏感，这进一步提示FAAH1可能调控着内源性大麻素信号通路(3)。与野生型小鼠相比，FAAH1缺失小鼠表现出对酒精的偏好，并且会自觉性增加对酒精的摄入，这提示FAAH1在调节成瘾行为中起着某些作用(1)。

1. Blednov, Y.A. et al. (2007) Neuropsychopharmacology 32, 1570-82.
2. Cravatt, B.F. et al. (1996) Nature 384, 83-7.
3. Cravatt, B.F. et al. (2001) Proc Natl Acad Sci USA 98, 9371-6.

原厂资料：

Specificity / Sensitivity

FAAH1 Antibody (Rat Specific) detects endogenous levels of total FAAH1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of rat FAAH1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Endogenous cannabinoids have been implicated in addictive behaviors and drug abuse (1). Fatty-acid amide hydrolase 1 (FAAH1) is a plasma membrane-bound hydrolase that converts oleamide to oleic acid (2). This hydrolase also converts the cannabinoid anandamide, the endogenous ligand for the CB1 cannabinoid receptor, to arachidonic acid, suggesting a role in fatty-acid amide inactivation (2). Mice lacking FAAH1 have significantly higher levels of anandamide in the brain and show decreased sensitivity to pain, further indicating a role for FAAH1 in the regulation of endocannabinoid signaling in vivo (3). FAAH1 null mice also demonstrate an increased preference for alcohol and an increased voluntary uptake of alcohol as compared to wild-type mice, indicating a role of FAAH1 in modulating addictive behaviors (1).

1. Blednov, Y.A. et al. (2007) Neuropsychopharmacology 32, 1570-82.
2. Cravatt, B.F. et al. (1996) Nature 384, 83-7.
3. Cravatt, B.F. et al. (2001) Proc Natl Acad Sci USA 98, 9371-6.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.