PhosphoPlus? Stat1 (Tyr701) Antibody Kit

• 产品编号：CST-9170S      品牌：CST       原厂货号：9170S
• 产品分类：生化和细胞分析 > 细胞分析试剂盒 > 细胞通路检测试剂盒
• 应用分类：

其它组分：

描述：

Phospho-Stat1 (Tyr701)抗体只能检测内源的在tyr701位点磷酸的Stat1蛋白。抗体能够检测tyr701位点磷酸化的p91 和 p84两种剪切形式。抗体不与对应的磷酸化残基的其它Stat 蛋白反应。Stat1 抗体能够检测内源水平Stat1 α (91kDa) 和 Stat1 β (84kDa) 总体蛋白水平。Phospho-Stat1 (Tyr701) 抗体是通过合成人源对应的Stat1 Tyr701位点周围的磷肽段来免疫动物而获得。Stat1 抗体是通过合成人源Stat1的序列的多肽来免疫动物而获得。抗体是通过protein A和多肽亲和层析法纯化。Stat1 转录因子的激活是通过一系列的配体完成 (1) 这对IFN-α和 IFN-γ的响应非常重要(2,3)。 在 Tyr701位点的磷酸化诱导 Stat1的形成二聚体, 入核和DNA结合(4)。 Stat1 蛋白是以成对的形式存在Stat1α (91 kDa) 和 另外的剪切方式Stat1β (84 kDa)。在很多细胞中，这两种形式都是通过IFN-α激活。但是有时 Stat1α是被 IFN-γ激活。 Stat1这种不适当的激活方式在存在于很多肿瘤中(5)。除了酪氨酸磷酸化，Stat1 也能通过p38有丝分裂激活蛋白激酶依赖的通路在Ser727位点磷酸化而激活，从而响应IFN-α 和其它的细胞胁迫(6)。丝氨酸磷酸化对最大化的诱导Stat1介导的基因激活也是必需的。

1. Heim, M.H. (1999) J Recept Signal Transduct Res 19, 75-120.
2. Durbin, J.E. et al. (1996) Cell 84, 443-50.
3. Meraz, M.A. et al. (1996) Cell 84, 431-42.
4. Ihle, J.N. et al. (1994) Trends Biochem Sci 19, 222-7.
5. Frank, D.A. (1999) Mol Med 5, 432-56.
6. Wen, Z. et al. (1995) Cell 82, 241-50.

原厂资料：

Specificity / Sensitivity

Phospho-Stat1 (Tyr701) antibody detects endogenous levels of Stat1 only when phosphorylated at Tyr701. This antibody detects phosphorylated tyrosine 701 of p91 Stat1 and also the p85 splice variant. It does not appreciably cross-react with the corresponding phosphorylated tyrosine of other Stat proteins. Stat1 antibody detects endogenous levels of total Stat1 α (91kDa) and Stat1 β (84kDa) protein.

Source / Purification

Antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr701 of human Stat1 (Phospho-Stat1 (Tyr701) antibody), or with a synthetic peptide corresponding to a sequence of human Stat1 (Stat1 antibody). Antibodies are purified by protein A and peptide affinity chromatography.

Background

The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.

1. Heim, M.H. (1999) J Recept Signal Transduct Res 19, 75-120.
2. Durbin, J.E. et al. (1996) Cell 84, 443-50.
3. Meraz, M.A. et al. (1996) Cell 84, 431-42.
4. Ihle, J.N. et al. (1994) Trends Biochem Sci 19, 222-7.
5. Frank, D.A. (1999) Mol Med 5, 432-56.
6. Wen, Z. et al. (1995) Cell 82, 241-50.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibodies.