Phospho-SEK1/MKK4 (Thr261) Antibody

• 产品编号：CST-9151S      品牌：CST       原厂货号：9151S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-SEK1/MKK4 (Thr261) Antibody兔多抗能检测内源性Thr261位点磷酸化的SEK1/MKK4蛋白水平。该抗体不与MEK1, MEK2和MKK3蛋白相应磷酸化残基发生交叉反应。该多克隆抗体通过使用与人源SEK1蛋白Thr261位点周围残基相一致的合成磷酸化肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。SAPK/Erk 激酶(SEK1)，也被称为MKK4或Jun激酶的激酶 (JNKK)，能激活MAPK同系物SAPK、 JNK，以应答各种细胞应激反应和炎性细胞因子(1-3)。SEK1的活化，是通过MEKK对其Ser257位点和Thr261位点的磷酸化实现的。类似于MEK，SEK也是一种双特异性蛋白激酶，能将SAPK/JNK活化环内的保守位点T*PY* 磷酸化(4)。Akt对SEK1的Ser80位点磷酸化作用，能够抑制SEK1以及应激活化的信号转导(5)。

1. Davis, R.J. (1994) Trends Biochem. Sci. 19, 470-473.
2. Sanchez, I. et al. (1994) Nature 372, 794-798.
3. Yan, M. et al. (1994) Nature 372, 798-800.
4. Kyriakis, J.M. et al. (1994) Nature 369, 156-160.
5. Park, H. et al. (2002) J. Biol. Chem. 277, 2573-2578.

原厂资料：

Specificity / Sensitivity

Phospho-SEK1/MKK4 (Thr261) Antibody detects endogenous levels of SEK1/MKK4 protein only when phosphorylated at threonine 261. This antibody does not cross-react with the corresponding phosphorylated residues of MEK1, MEK2 or MKK3.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Thr261 of human SEK1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

SAPK/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflamatory cytokines (1-3). Activation of SEK1 occurs through MEKK phosphorylation of serine and threonine residues at positions 257 and 261, respectively. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop (4). Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses stress-activated signal transduction (5).

1. Davis, R.J. (1994) Trends Biochem. Sci. 19, 470-473.
2. Sanchez, I. et al. (1994) Nature 372, 794-798.
3. Yan, M. et al. (1994) Nature 372, 798-800.
4. Kyriakis, J.M. et al. (1994) Nature 369, 156-160.
5. Park, H. et al. (2002) J. Biol. Chem. 277, 2573-2578.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.