HEXIM1 Antibody

• 产品编号：CST-9064S      品牌：Cell Signaling Technology       原厂货号：9064S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

HEXIM1 Antibody能够检测内源性的HEXIM1总蛋白水平。该多克隆抗体是采用合成的与人源HEXIM1蛋白氨基端周围相应的多肽片段免疫动物而生产的。通过蛋白A和多肽亲和层析纯化获得。Hexamethylene bisacetamide-induced protein 1 (HEXIM1)起初被鉴定在血管平滑肌中的一个蛋白，该蛋白在hexamethylene bisacetamide分化试剂的刺激下被上调(1)。HEXIM1结合到一个高丰度的非编码RNA 7SK RNA，并且一起作为positive transcription elongation b (P-TEFb)的强有力的抑制剂(2,3)。P-TEFb蛋白可使RNA polymerase II 的最大亚基的羧基端区域磷酸化，以及使一个重要的转录延伸因子的调节蛋白(4-8)。7SK RNA结合的HEXIM1可与P-TEFb蛋白的Cyclin T1 亚单位相互作用，这隔绝它在一个非活性形式，从而导致转录起始(2,3)。在细胞中P-TEFb蛋白非活性形式与活性形式的相对比例通过HEXIM1/7SK RNA的调节被认为在广泛细胞内基因表达进程中起着关键作用，例如雌激素和糖皮质激素受体调节的基因(9-12)。

1. Ouchida, R. et al. (2003) Genes Cells 8, 95-107.
2. Michels, A.A. et al. (2004) EMBO J 23, 2608-19.
3. Yik, J.H. et al. (2003) Mol Cell 12, 971-82.
4. Buratowski, S. (2009) Mol Cell 36, 541-6.
5. Lenasi, T. and Barboric, M. RNA Biol 7, 145-50.
6. Pirngruber, J. et al. (2009) Cell Cycle 8, 3636-42.
7. Wada, T. et al. (1998) EMBO J 17, 7395-403.
8. Yamada, T. et al. (2006) Mol Cell 21, 227-37.
9. Peterlin, B.M. et al. (2012) Wiley Interdiscip Rev RNA 3, 92-103.
10. Ketchart, W. et al. (2011) Oncogene 30, 3563-9.
11. Ogba, N. et al. (2008) Cancer Res 68, 7015-24.
12. Shimizu, N. et al. (2005) Proc Natl Acad Sci U S A 102, 8555-60.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Bovine, Dog, Horse

Specificity / Sensitivity

HEXIM1 Antibody recognizes endogenous levels of total HEXIM1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human HEXIM1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Hexamethylene bisacetamide-induced protein 1 (HEXIM1) was originally identified in vascular smooth muscle cells as a protein that is upregulated upon treatment with the differentiating agent hexamethylene bisacetamide (1). HEXIM1 binds to 7SK RNA, a highly abundant non-coding RNA, and together act as a potent inhibitor of positive transcription elongation b (P-TEFb) (2,3). P-TEFb phosphorylates the C-terminal domain of the largest subunit of RNA polymerase II and is an important regulator of transcription elongation (4-8). 7SK RNA-bound HEXIM1 interacts with the Cyclin T1 subunit of P-TEFb, sequestering it in an inactive form leading to transcription inhibition (2,3). The regulation of the relative ratio of inactive to active P-TEFb in the cell by HEXIM1/7SK RNA is thought to play a critical role in regulation of a wide range of cellular gene expression programs such as estrogen and glucocorticoid receptor regulated genes (9-12).

1. Ouchida, R. et al. (2003) Genes Cells 8, 95-107.
2. Michels, A.A. et al. (2004) EMBO J 23, 2608-19.
3. Yik, J.H. et al. (2003) Mol Cell 12, 971-82.
4. Buratowski, S. (2009) Mol Cell 36, 541-6.
5. Lenasi, T. and Barboric, M. RNA Biol 7, 145-50.
6. Pirngruber, J. et al. (2009) Cell Cycle 8, 3636-42.
7. Wada, T. et al. (1998) EMBO J 17, 7395-403.
8. Yamada, T. et al. (2006) Mol Cell 21, 227-37.
9. Peterlin, B.M. et al. (2012) Wiley Interdiscip Rev RNA 3, 92-103.
10. Ketchart, W. et al. (2011) Oncogene 30, 3563-9.
11. Ogba, N. et al. (2008) Cancer Res 68, 7015-24.
12. Shimizu, N. et al. (2005) Proc Natl Acad Sci U S A 102, 8555-60.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.