SMARCC1/BAF155 Antibody

• 产品编号：CST-9053S      品牌：Cell Signaling Technology       原厂货号：9053S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SMARCC1/BAF155 Antibody能够检测内源性SMARCC1/BAF155总蛋白水平。该多克隆抗体是采用合成的与人源SMARCC1/BAF155蛋白Ala202残基周围序列相对应的肽段免疫动物而生产的。通过蛋白A和多肽亲和层析纯化抗体获得。

ATP依赖的染色质修饰复合物在调节细胞核进程中起着重要的作用，例如转录、DNA复制和修复(1,2)。SWI/SNF染色质修饰复合物是有超过10个亚单位组成，并且包含BRM或BRG1的一个单独分子，这两个蛋白作为ATPase催化亚单位。ATPase亚单位的激活干扰组蛋白-DNA相互作用，并且改变了染色质重要调节元件的接近能力。另外中心的和附加的亚单位起到支撑作用去维持稳定性，并且提供了界面为不同转录因子和染色质相互作用(2-5)。SWI/SNF亚单位和转录因子例如细胞核受体、p53、Rb、BRCA1、MyoD之间的相互作用有助于复合物招募到靶基因去调节基因激活、细胞生长、细胞周期和分化进程(1,6-9)。

SMARCC1/BAF155是一个SWI/SNF复合物的中心亚单位，该蛋白在体外对有效地核小体修饰是必须的(10)。SMARCC1是小鼠胚胎干细胞特定的SWI/SNF复合物(esBAF)的一个重要部分，它对于早期胚胎发育以及正确的大脑和血清内胚层发育是必须的(11-13)。

1. Ho, L. and Crabtree, G.R. (2010) Nature 463, 474-84.
2. Becker, P.B. and Hörz, W. (2002) Annu Rev Biochem 71, 247-73.
3. Eberharter, A. and Becker, P.B. (2004) J Cell Sci 117, 3707-11.
4. Bowman, G.D. (2010) Curr Opin Struct Biol 20, 73-81.
5. Gangaraju, V.K. and Bartholomew, B. (2007) Mutat Res 618, 3-17.
6. Lessard, J.A. and Crabtree, G.R. (2010) Annu Rev Cell Dev Biol 26, 503-32.
7. Morettini, S. et al. (2008) Front Biosci 13, 5522-32.
8. Wolf, I.M. et al. (2008) J Cell Biochem 104, 1580-6.
9. Simone, C. (2006) J Cell Physiol 207, 309-14.
10. Phelan, M.L. et al. (1999) Mol Cell 3, 247-53.
11. Han, D. et al. (2008) Dev Biol 315, 136-46.
12. Kim, J.K. et al. (2001) Mol Cell Biol 21, 7787-95.
13. Schaniel, C. et al. (2009) Stem Cells 27, 2979-91.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Dog

Specificity / Sensitivity

SMARCC1/BAF155 Antibody recognizes endogenous levels of total SMARCC1/BAF155 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala202 of human SMARCC1/BAF155 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

ATP-dependent chromatin remodeling complexes play an essential role in the regulation of nuclear processes such as transcription and DNA replication and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits and contains a single molecule of either BRM or BRG1 as the ATPase catalytic subunit. The activity of the ATPase subunit disrupts histone-DNA contacts and changes the accessibility of crucial regulatory elements to the chromatin. The additional core and accessory subunits play a scaffolding role to maintain stability and provide surfaces for interaction with various transcription factors and chromatin (2-5). The interactions between SWI/SNF subunits and transcription factors such as nuclear receptors, p53, Rb, BRCA1, and MyoD facilitate recruitment of the complex to target genes to regulate gene activation, cell growth, cell cycle, and differentiation processes (1,6-9).

SMARCC1/BAF155 is one of the core subunits of the SWI/SNF complex, which is necessary for efficient nucleosome remodeling by BRG1 in vitro (10). SMARCC1 is an essential part of the mouse embryonic stem cell specific SWI/SNF complex (esBAF), which is necessary for early embryogenesis and proper brain and visceral endoderm development (11-13).

1. Ho, L. and Crabtree, G.R. (2010) Nature 463, 474-84.
2. Becker, P.B. and Hörz, W. (2002) Annu Rev Biochem 71, 247-73.
3. Eberharter, A. and Becker, P.B. (2004) J Cell Sci 117, 3707-11.
4. Bowman, G.D. (2010) Curr Opin Struct Biol 20, 73-81.
5. Gangaraju, V.K. and Bartholomew, B. (2007) Mutat Res 618, 3-17.
6. Lessard, J.A. and Crabtree, G.R. (2010) Annu Rev Cell Dev Biol 26, 503-32.
7. Morettini, S. et al. (2008) Front Biosci 13, 5522-32.
8. Wolf, I.M. et al. (2008) J Cell Biochem 104, 1580-6.
9. Simone, C. (2006) J Cell Physiol 207, 309-14.
10. Phelan, M.L. et al. (1999) Mol Cell 3, 247-53.
11. Han, D. et al. (2008) Dev Biol 315, 136-46.
12. Kim, J.K. et al. (2001) Mol Cell Biol 21, 7787-95.
13. Schaniel, C. et al. (2009) Stem Cells 27, 2979-91.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody