Dasatinib

• 产品编号：CST-9052S      品牌：CST       原厂货号：9052S
• 产品分类：生化试剂 > 生物试剂 > 生物活性小分子 > 抑制剂
• 应用分类：

描述：

提供的达沙替尼为冻干粉。对于5mM浓度贮存液，在5mg冻干粉中加入2.05 ml DMSO。工作浓度和处理时间根据所需的预期效果来确定，一般是50-500 nM，处理2-6小时。能溶于DMSO（200 mg/ml），难溶于乙醇和水，在水中的最大溶解度约为10 μM。达沙替尼是一种Src和Bcr-Abl酪氨酸激酶的小分子抑制剂，针对这两种激酶的IC50值分别为0.55和3.0nm（1）。伊马替尼和达沙替尼有相似的分子机制，但是达沙替尼对伊马替尼耐药的细胞系有较强的作用，并且对血小板衍生生长因子（PDGF）有较强的抑制作用（2,3）。

原厂资料：

Molecular Characterization

C22H26ClN7O2S

Purity

>99%

Directions for Use

Dasatinib is supplied as a lyophilized powder. For a 5 mM stock, reconstitute the 5 mg in 2.05 mL DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 50-500 nM for 2-6 hours. Soluble in DMSO at 200 mg/mL; very poorly soluble in ethanol and water with maximum solubility in water estimated to be about 10 μM.

Background

Dasatinib is a small molecule inhibitor of both the Src and Bcr-Abl tyrosine kinases, with IC50 values of 0.55 and 3.0 nM for the isolated kinases, respectively (1). Imatinib and dasatinib have similar inhibitory mechanisms, but dasatinib displays greater potency in imatinib-resistant cell lines and greater inhibitory effect on platelet derived growth factor (PDGF) (2,3).

注意事项：

Storage: Store lyophilized or in solution at -20°C, desiccated.
Protect from light. In lyophilized form, the chemical is stable for
24 months. Once in solution, use within 3 months to prevent loss
of potency. Aliquot to avoid multiple freeze/thaw cycles.