Phospho-CDCP1 (Tyr734) Antibody

• 产品编号：CST-9050S      品牌：CST       原厂货号：9050S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-CDCP1 (Tyr734) Antibody 可以识别734位酪氨酸磷酸化的内源性CDCP1蛋白。该抗体也可以与分子量为100 kDa的其他尚未明确的蛋白发生交叉反应。该多克隆抗体是由针对人CDCP1蛋白734位酪氨酸磷酸化附近的磷酸肽免疫动物而产生。抗体由protein A和肽亲和层析技术纯化。CDCP1(CUB结构域包含蛋白, SIMA135)可能是一种干细胞的标记物，于多种研究中被发现在人癌细胞、典型和非典型结肠癌中高度表达（1）。CDCP1的表达可能受到表观遗传学调节，因为启动子CpG序列的甲基化可以导致CDCP1表达减少（2）。CDCP1的基因也编码由多种结构域形成的跨膜糖蛋白复合物抗原。3种胞外区的CUB结构域有可能在细胞粘附或细胞外基质相互作用中起作用（1,3）。Src-family kinases可能磷酸化CDCP1胞浆区第5位的酪氨酸残基从而为包含SH2结构域的蛋白提供结合位点（3）。CDCP1可能是一种造血干细胞标记物(4,5)。

1. Scherl-Mostageer, M. et al. (2001) Oncogene 20, 4402-8.
2. Ikeda, J.I. et al. (2006) J. Pathol. 210, 75-84.
3. Hooper, J.D. et al. (2003) Oncogene 22, 1783-94.
4. Conze, T. et al. (2003) Ann. N.Y. Acad. Sci. 996, 222-226.
5. Bühring, H.J. et al. (2004) Stem Cells 22, 334-343.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey

Specificity / Sensitivity

Phospho-CDCP1 (Tyr734) Antibody recognizes endogenous levels of CDCP1 protein only when phosphorylated at Tyr734. This antibody also cross-reacts with an unidentified protein of 100 kDa.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr734 of human CDCP1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

CDCP1 (CUB domain containing protein, SIMA135) is a putative stem cell marker that has been shown in research studies to be highly expressed in some human cancer cells and in both typical and atypical (cancerous) colons (1). Expression of CDCP1 may be epigenetically regulated, as methylation of promoter CpG sequences results in decreased CDCP1 expression (2). The corresponding CDCP1 gene encodes a glycoprotein that acts as a complex, multidomain transmembrane antigen. Three CUB domains in its extracellular region are possibly involved in cell adhesion or extracellular matrix interaction (1,3). Src-family kinases may phosphorylate CDCP1 at five tyrosine residues within its cytoplasmic domain to provide a potential binding site for SH2 domain-containing proteins (3). CDCP1 is a putative marker for hematopoietic stem cells (4,5).

1. Scherl-Mostageer, M. et al. (2001) Oncogene 20, 4402-8.
2. Ikeda, J.I. et al. (2006) J. Pathol. 210, 75-84.
3. Hooper, J.D. et al. (2003) Oncogene 22, 1783-94.
4. Conze, T. et al. (2003) Ann. N.Y. Acad. Sci. 996, 222-226.
5. Bühring, H.J. et al. (2004) Stem Cells 22, 334-343.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.