Insulin (C27C9) Rabbit mAb (Alexa Fluor? 647 Conjugate)

• 产品编号：CST-9008S      品牌：CST       原厂货号：9008S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Insulin (C27C9) Rabbit mAb兔单抗 (Alexa Fluor® 647 Conjugate)能够检测内源性的胰岛素总蛋白水平。该单克隆抗体是由合成的人源胰岛素肽段免疫动物而制备的。这款CST抗体标记了Alexa Fluor® 647荧光染料，通过了内部测试可以直接用于大鼠细胞的免疫荧光分析以及人和小鼠细胞的流式试验。这款抗体与不带标记Insulin (C27C9) Rabbit mAb 兔单抗#3014有着相同的种属交叉反应。葡萄糖稳态的维持是一个激素调节的重要生理过程。在进食过程中血糖水平的升高，会经由葡萄糖感应通路（1）刺激胰岛β细胞释放胰岛素。胰岛素的前体是胰岛素原，它在分泌之前被进一步加工。 其中A-和B-肽通过二硫键连接在一起以形成胰岛素，而前体分子的中间部分肽段被裂解并释放，称为C肽。胰岛素能刺激骨骼肌和脂肪组织从血液中摄取葡萄糖。胰岛素缺乏会导致1型糖尿病（2）。

1. Straub, S.G. and Sharp, G.W. (2002) Diabetes Metab. Res. Rev. 18, 451-463.
2. Concannon, P. et al. (1998) Nat. Genet. 19, 292-296.

原厂资料：

Specificity / Sensitivity

Insulin (C27C9) Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total insulin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human insulin.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct immunofluorescent analysis in rat cells and flow cytometry in human and mouse cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Insulin (C27C9) Rabbit mAb #3014.

Background

The maintenance of glucose homeostasis is an essential physiological process that is regulated by hormones. An elevation in blood glucose levels during feeding stimulates insulin release from pancreatic β cells through a glucose sensing pathway (1). Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form insulin, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Insulin stimulates glucose uptake from blood into skeletal muscle and adipose tissue. Insulin deficiency leads to type 1 diabetes mellitus (2).

1. Straub, S.G. and Sharp, G.W. (2002) Diabetes Metab. Res. Rev. 18, 451-463.
2. Concannon, P. et al. (1998) Nat. Genet. 19, 292-296.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the anti-
body. Protect from light. Do not freeze.