Reptin/RuvBL2 Antibody

• 产品编号：CST-8959S      品牌：CST       原厂货号：8959S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Reptin/RuvBL2 Antibody能够检测内源性Reptin/RuvBL2总蛋白水平。通过合成的与人源Reptin/RuvBL2蛋白Gly263位点相应多肽片段去免疫动物从而制备出此多克隆抗体。通过蛋白A和多肽亲和层析纯化获得。Reptin/RuvBL2和Pontin/RuvBL1是与ATPase密切相关的成员，而ATPase是与多样的细胞内活动(AAA+)蛋白质的超家族有关，这些蛋白质显示了与细菌RuvB蛋白的同源性，RuvB蛋白可促进霍利迪连结体的分支迁移法(1)。两个蛋白能够一起被发现作为染色体重塑和修饰复合物的一部分，例如INO80、TIP60、SRCAP和Uri1，这些复合物都涉及到转录调控(1,2)。这些复合物中的成员Reptin涉及到由c-Myc、β-catenin和E1A驱动的致癌的转化，而这些作为一个本质的靶基因转录辅助调节因子。Reptin也在调节细胞内缺氧反应中起着重要作用。低氧可诱导Reptin甲基化，这是通过甲基转移酶G9a导致它招募到缺氧反应启动子上，在那里它能负性调节这些基因的转录(8)。除了转录调控角色之外，Reptin也参与端粒酶生物合成过程，因为是端粒酶复合物的一部分，并且它参与DNA损伤反应，因为是TIP60乙酰转移酶复合物的一部分，该复合物可刺激ATM激酶活性，这对于涉及检测点激活和DNA修复蛋白质的磷酸化是必须的(9,10)。

1. Jha, S. and Dutta, A. (2009) Mol Cell 34, 521-33.
2. Gallant, P. (2007) Trends Cell Biol 17, 187-92.
3. Huber, O. et al. (2008) Cancer Res 68, 6873-6.
4. Kim, J.H. et al. (2005) Nature 434, 921-6.
5. Bauer, A. et al. (2000) EMBO J 19, 6121-30.
6. Wood, M.A. et al. (2000) Mol Cell 5, 321-30.
7. Dugan, K.A. et al. (2002) Oncogene 21, 5835-43.
8. Lee, J.S. et al. (2010) Mol Cell 39, 71-85.
9. Venteicher, A.S. et al. (2008) Cell 132, 945-57.
10. Sun, Y. et al. (2010) Cell Cycle 9, 930-6.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Xenopus, Zebrafish, Bovine, Dog

Specificity / Sensitivity

Reptin/RuvBL2 Antibody recognizes endogenous levels of total Reptin/RuvBL2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly263 of human Reptin/RuvBL2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Reptin/RuvBL2 and Pontin/RuvBL1 are closely related members of the ATPase Associated with diverse cellular Activities (AAA+) super family of proteins that show homology to bacterial RuvB that drives branch migration of Holliday junctions (1). Both proteins can be found together as part of chromatin remodeling and modification complexes such as INO80, TIP60, SRCAP, and Uri1 that are involved in transcription regulation (1,2). Reptin, as part of these complexes, has been implicated in oncogenic transformation driven by c-Myc, β-catenin, and E1A by acting as an essential transcriptional coregulator of target genes (2-7). Reptin also plays a role in modulating cellular responses to hypoxia. Hypoxia induced methylation of Reptin by the methyltransferase G9a leads to its recruitment to hypoxia responsive promoters where it negatively regulates transcription of these genes (8). In addition to transcriptional regulatory roles, Reptin also participates in the telomerase biogenesis processes as part of the telomerase complex and in DNA damage response as part of the TIP60 acetyltransferase complex that stimulates ATM kinase activity necessary for phosphorylation of proteins involved in both checkpoint activation and DNA repair (9,10).

1. Jha, S. and Dutta, A. (2009) Mol Cell 34, 521-33.
2. Gallant, P. (2007) Trends Cell Biol 17, 187-92.
3. Huber, O. et al. (2008) Cancer Res 68, 6873-6.
4. Kim, J.H. et al. (2005) Nature 434, 921-6.
5. Bauer, A. et al. (2000) EMBO J 19, 6121-30.
6. Wood, M.A. et al. (2000) Mol Cell 5, 321-30.
7. Dugan, K.A. et al. (2002) Oncogene 21, 5835-43.
8. Lee, J.S. et al. (2010) Mol Cell 39, 71-85.
9. Venteicher, A.S. et al. (2008) Cell 132, 945-57.
10. Sun, Y. et al. (2010) Cell Cycle 9, 930-6.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.