Filamin B Antibody

• 产品编号：CST-8949S      品牌：CST       原厂货号：8949S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Filamin B抗体可以识别内源性总的Filamin B蛋白。抗体会检测到一条100kDa未知源性的条带。基于氨基酸序列，该抗体与Filamin A或Filamin C没有交叉反应。多抗由合成肽段免疫动物产生，该肽段与人filamin B蛋白羧基端肽段序列一致。抗体由蛋白A和肽段亲和层析技术纯化得到。纤维状蛋白是一类二聚体actin结合蛋白，可以作为细胞黏附位点的组成部分。它们也作为折叠蛋白成为信号分子的亚细胞靶点（1）。Actin结合结构域（alpha-actinin）位于氨基末端后面接着是96个氨基酸残基组成的24个串联重复序列，二聚体结构域位于羧基端。除了actin 微丝，纤维状蛋白也可以和其他结构和信号分子，例如beta整合素，Rho/Rac/Cdc42, PKC,和胰岛素受体，通过羧基端二聚体结构域结合（1-3）。纤维状蛋白A，表达丰度最高，和纤维状蛋白B都是广泛表达的亚型，纤维状蛋白C主要表达在肌肉中（1）。纤维状蛋白A能被PAK1在2152位丝氨酸磷酸化，这对PAK1介导的actin细胞骨架重构是必须的（4）。

1. Stossel, T.P. et al. (2001) Nat Rev Mol Cell Biol 2, 138-45.
2. Tigges, U. et al. (2003) J Biol Chem 278, 23561-9.
3. He, H.J. et al. (2003) J Biol Chem 278, 27096-104.
4. Vadlamudi, R.K. et al. (2002) Nat Cell Biol 4, 681-90.

原厂资料：

Specificity / Sensitivity

Filamin B Antibody recognizes endogenous levels of total filamin B protein. This antibody detects a 100 kDa background band of unknown origin. Based on the amino acid sequence, this antibody is not expected to cross-react with filamin A or filamin C.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human filamin B protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Filamins are a family of dimeric actin binding proteins that function as structural components of cell adhesion sites. They also serve as a scaffold for subcellular targeting of signaling molecules (1). The actin binding domain (alpha-actinin domain) located at the amino terminus is followed by as many as 24 tandem repeats of about 96 residues and the dimerization domain is located at the carboxy terminus. In addition to actin filaments, filamins associate with other structural and signaling molecules such as beta integrins, Rho/Rac/Cdc42, PKC, and the insulin receptor, primarily through the carboxy-terminal dimerization domain (1-3). Filamin A, the most abundant, and filamin B are widely expressed isoforms, while filamin C is predominantly expressed in muscle (1). Filamin A is phosphorylated by PAK1 at Ser2152, which is required for PAK1-mediated actin cytoskeleton reorganization (4).

1. Stossel, T.P. et al. (2001) Nat Rev Mol Cell Biol 2, 138-45.
2. Tigges, U. et al. (2003) J Biol Chem 278, 23561-9.
3. He, H.J. et al. (2003) J Biol Chem 278, 27096-104.
4. Vadlamudi, R.K. et al. (2002) Nat Cell Biol 4, 681-90.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.