PRAS40 (D23C7) XP? Rabbit mAb (Biotinylated)

• 产品编号：CST-8858S      品牌：CST       原厂货号：8858S
• 产品分类：抗体 > 一抗 > 表位标签和报告基因特异性抗体
• 应用分类：

描述：

PRAS40 (D23C7) XP® Rabbit mAb (Biotinylated) 兔单抗可以检测内源性的总PRAS40。该单克隆抗体是由针对人源PRAS40 蛋白氨基酸序列的合成肽段免疫动物而生产的。CST公司的本抗体在最佳条件下结合了生物素，该生物素化的抗体同未标记的PRAS40 (D23C7) XP ® Rabbit mAb #2691一样，呈现出相同物种间的交叉反应。许多生长因子和激素诱导PI3K激酶信号通路，从而激活下游效应蛋白，例如丝氨酸/苏氨酸激酶Akt（1,2）。一个已知的Akt的底物是一个40 kDa的，富含脯氨酸的蛋白（PRAS40），可以结合14-3-3蛋白（2）。PRAS40还能够结合mTOR，传递Akt信号到mTOR复合物。抑制mTOR信号可以刺激PRAS40结合到mTOR上，反过来抑制mTOR的活性（3）。在缺失胰岛素的细胞内PRAS40和mTOR复合物1（mTORC1）中的Raptor 结合，进而抑制细胞周期蛋白Rheb调节的mTORC1信号通路的激活。Akt磷酸化PRAS40的Thr246位点，解除PRAS40对mTORC1的抑制（4）。反过来mTORC1磷酸化PRAS40的Ser183（5）。

1. Cantley, L.C. (2002) Science 296, 1655-7.
2. Kovacina, K.S. et al. (2003) J Biol Chem 278, 10189-94.
3. Vander Haar, E. et al. (2007) Nat Cell Biol 9, 316-23.
4. Sancak, Y. et al. (2007) Mol Cell 25, 903-15.
5. Oshiro, N. et al. (2007) J Biol Chem 282, 20329-39.

原厂资料：

Specificity / Sensitivity

PRAS40 (D23C7) XP® Rabbit mAb (Biotinylated) detects endogenous levels of total PRAS40 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PRAS40 protein.

Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated PRAS40 (D23C7) XP® Rabbit mAb #2691.

Background

Many growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which results in the activation of downstream effector proteins such as the serine/threonine kinase Akt (1,2). One known Akt substrate is a 40 kDa, proline-rich protein (PRAS40) that binds to 14-3-3 proteins (2). PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex. Inhibition of mTOR signaling stimulates PRAS40 binding to mTOR, which in turn inhibits mTOR activity (3). PRAS40 interacts with raptor in mTOR complex 1 (mTORC1) in insulin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1 (4). mTORC1 in turn phosphorylates PRAS40 at Ser183 (5).

1. Cantley, L.C. (2002) Science 296, 1655-7.
2. Kovacina, K.S. et al. (2003) J Biol Chem 278, 10189-94.
3. Vander Haar, E. et al. (2007) Nat Cell Biol 9, 316-23.
4. Sancak, Y. et al. (2007) Mol Cell 25, 903-15.
5. Oshiro, N. et al. (2007) J Biol Chem 282, 20329-39.

注意事项：

Storage: Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM
sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA and 50%
glycerol. Store at -20ºC. Do not aliquot the antibody.