Myosin IIb (D8H8) XP? Rabbit mAb

• 产品编号：CST-8824S      品牌：CST       原厂货号：8824S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Myosin IIb (D8H8) XP® Rabbit mAb可以识别内源性总的myosin IIb蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人myosin IIb蛋白羧基末端附近氨基酸一致。非肌肉肌球蛋白是一个actin-based马达蛋白，对于细胞运动，细胞分裂，迁移，黏附和极化都非常重要。该holoenzyme由2个相同的重链和2组轻链组成。轻链（MLCs）调控肌球蛋白II的活性和稳定性。重链（NMHCs）由3个基因编码，MYH9, MYH10, 和MYH14,会形成3种不同的非肌肉肌球蛋白II，分别是IIa, IIb, 和IIc（1）。3个亚型都发挥同样的酶活作用，与收缩的actin微丝结合协助ATP水解，它们的细胞功能似乎并不多余，三个亚型有不同的亚细胞分布（2-5）。肌球蛋白II的羧基末端对于亚型特异性的亚细胞分布非常重要（60。研究表明肌球蛋白II的1943位丝氨酸磷酸化有助于对乳腺癌细胞的迁移进行调控（7）。

1. Conti, M.A. and Adelstein, R.S. (2008) J Cell Sci 121, 11-18.
2. Sandquist, J.C. et al. (2006) J Biol Chem 281, 35873-83.
3. Even-Ram, S. et al. (2007) Nat Cell Biol 9, 299-309.
4. Vicente-Manzanares, M. et al. (2007) J Cell Biol 176, 573-80.
5. Wylie, S.R. and Chantler, P.D. (2008) Mol Biol Cell 19, 3956-68.
6. Sandquist, J.C. and Means, A.R. (2008) Mol Biol Cell 19, 5156-67.
7. Dulyaninova, N.G. et al. (2007) Mol Biol Cell 18, 3144-55.

原厂资料：

Specificity / Sensitivity

Myosin IIb (D8H8) XP® Rabbit mAb recognizes endogenous levels of total myosin IIb protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human myosin IIb protein.

Background

Nonmuscle myosin is an actin-based motor protein essential to cell motility, cell division, migration, adhesion, and polarity. The holoenzyme consists of two identical heavy chains and two sets of light chains. The light chains (MLCs) regulate myosin II activity and stability. The heavy chains (NMHCs) are encoded by three genes, MYH9, MYH10, and MYH14, which generate three different nonmuscle myosin II isoforms, IIa, IIb, and IIc, respectively (reviewed in 1). While all three isoforms perform the same enzymatic tasks, binding to and contracting actin filaments coupled to ATP hydrolysis, their cellular functions do not appear to be redundant and they have different subcellular distributions (2-5). The carboxy-terminal tail domain of myosin II is important in isoform-specific subcellular localization (6). Research studies have shown that phosphorylation of myosin IIa at Ser1943 contributes to the regulation of breast cancer cell migration (7).

1. Conti, M.A. and Adelstein, R.S. (2008) J Cell Sci 121, 11-18.
2. Sandquist, J.C. et al. (2006) J Biol Chem 281, 35873-83.
3. Even-Ram, S. et al. (2007) Nat Cell Biol 9, 299-309.
4. Vicente-Manzanares, M. et al. (2007) J Cell Biol 176, 573-80.
5. Wylie, S.R. and Chantler, P.D. (2008) Mol Biol Cell 19, 3956-68.
6. Sandquist, J.C. and Means, A.R. (2008) Mol Biol Cell 19, 5156-67.
7. Dulyaninova, N.G. et al. (2007) Mol Biol Cell 18, 3144-55.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody