LysoTracker? Green DND-26

• 产品编号：CST-8783S      品牌：CST       原厂货号：8783S
• 产品分类：生化试剂 > 染料/染色剂/指示剂/底物 > 细胞染料 > 其他
• 应用分类：

描述：

每小瓶LysoTracker® Green DND-26包含50 μl浓度为1mM的溶液（溶于DMSO）。直接在普通培养基中以1:20,000稀释，工作浓度为50 nM，然后立即进行分析。细胞必须实时成像，不要固定！为了减少培养基的背景荧光，在成像前用没有酚红的培养基代替普通培养基。绿色背景抑制剂，如CST公司的BackDrop™ Background Suppressor，也可以用来减少背景荧光。LysoTracker® Green DND-26是一种细胞可透过的绿色染料，能够对活细胞中的酸性区域（溶酶体）进行染色

原厂资料：

Directions for Use

Each vial of LysoTracker® Green DND-26 contains 50 μl of solution at a 1 mM concentration in DMSO. Dilute 1:20,000 directly into normal growth media for a working concentration of 50 nM and analyze immediately. Cells must be imaged live; DO NOT FIX! 
 To reduce background fluorescence of the media, phenol-red free media can be substituted for normal media prior to imaging. A green background suppressor, such as BackDrop™ Background Suppressor from Life Technologies, can also be added to reduce background fluorescence.

Background

LysoTracker® Green DND-26 is a cell permeable green dye that stains acidic compartments (lysosomes) in live cells.

注意事项：

Storage: Store at -20ºC dessicated and protected from light.
This reagent is stable for 6 months. Seal vial tightly after use to
refreeze. Avoid excessive freeze-thaw cycles.