Kinectin 1 Antibody

• 产品编号：CST-8772S      品牌：Cell Signaling Technology       原厂货号：8772S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

kinectin 1抗体可以识别内源性总的kinectin 1蛋白。多抗由合成肽段免疫动物产生，该肽段与人kinectin 1 protein蛋白氨基端邻近序列一致。抗体由蛋白A和肽段亲和层析技术纯化得到。Kinectin 1/KTN1是一个内质网（ER）富集整合膜蛋白可能涉及了ER的形成（1）。Kinectin 1与微管马达蛋白，kinesin结合，作为一个膜锚定蛋白为kinesin-base细胞器示踪发挥作用（2）。Kinesin和kinectin1的相互作用会影响内质网-支持的粘着斑聚集（3）。Kinectin1也在翻译延伸过程中发挥作用，作为延伸因子复合物与内质网结合的锚蛋白（4，5）。研究人员证明kinectin1的表达在多种人类病理状态下会受到影响，包括乳腺癌（6），肝癌（HCC）(7),帕金森病（8）和自身免疫综合征白塞氏病（BD）（9,10）。

1. Lin, S. et al. (2012) Int J Biochem Cell Biol 44, 1436-43.
2. Kumar, J. et al. (1995) Science 267, 1834-7.
3. Zhang, X. et al. (2010) J Cell Sci 123, 3901-12.
4. Ong, L.L. et al. (2003) J Biol Chem 278, 32115-23.
5. Ong, L.L. et al. (2006) J Biol Chem 281, 33621-34.
6. Sutton, C.W. et al. (2010) J Proteome Res 9, 3891-902.
7. Wang, H.C. et al. (2004) Biochem Cell Biol 82, 321-7.
8. van Dijk, K.D. et al. (2012) Brain Pathol 22, 485-98.
9. Lu, Y. et al. (2005) Arthritis Res Ther 7, R1133-9.
10. Feng, X.G. et al. (2007) Clin Exp Rheumatol 25, S80-5.

原厂资料：

Specificity / Sensitivity

Kinectin 1 Antibody recognizes endogenous levels of total kinectin 1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human kinectin 1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Kinectin 1/KTN1 is an endoplasmic reticulum (ER)-enriched integral membrane protein that may be involved in the formation of ER sheets (reviewed in 1). Kinectin 1 binds the microtubule motor protein, kinesin, and acts as a membrane anchor for kinesin-based organelle trafficking (2). The interaction of kinesin with kinectin 1 has been shown to affect ER-supported focal adhesion assembly (3). Kinectin 1 has also been implicated in translation elongation, as an anchor for the elongation factor complex to the ER (4,5). Research investigators have shown that kinectin 1 expression is altered in multiple human pathologies, including breast cancer (6), hepatocellular carcinoma (HCC) (7), Parkinson's disease (8), and the autoimmune syndrome Behçet's disease (BD) (9,10).

1. Lin, S. et al. (2012) Int J Biochem Cell Biol 44, 1436-43.
2. Kumar, J. et al. (1995) Science 267, 1834-7.
3. Zhang, X. et al. (2010) J Cell Sci 123, 3901-12.
4. Ong, L.L. et al. (2003) J Biol Chem 278, 32115-23.
5. Ong, L.L. et al. (2006) J Biol Chem 281, 33621-34.
6. Sutton, C.W. et al. (2010) J Proteome Res 9, 3891-902.
7. Wang, H.C. et al. (2004) Biochem Cell Biol 82, 321-7.
8. van Dijk, K.D. et al. (2012) Brain Pathol 22, 485-98.
9. Lu, Y. et al. (2005) Arthritis Res Ther 7, R1133-9.
10. Feng, X.G. et al. (2007) Clin Exp Rheumatol 25, S80-5.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody