Bid (3C5) Mouse mAb

• 产品编号：CST-8762S      品牌：CST       原厂货号：8762S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Bid (3C5) Mouse mAb 鼠单抗能够检测内源的 Bid 总蛋白。在凋亡过程中，此抗体不与内源的其它tBid蛋白交叉反应。该单克隆抗体是采用人源 Bid 蛋白的全长序列融合蛋白来免疫动物而获得。Bid是Bcl-2 家族促凋亡“只有 BH3 域” 成员之一，最初研究发现，其既与抗凋亡家族成员Bcl-2互作，又与促凋亡蛋白Bax互作(1)。Bid 通常以非活性前体形式存在于细胞质部分，在Fas信号通路中被caspase-8在Asp60位点剪切，导致其 C-末端p15片段(tBid)转运到线粒体外膜上(2-4)。Bid 的转运及重新定位与线粒体细胞色素C的释放有关，导致其与Apaf-1和caspase-9 形成复合体并激活caspase-9 (5-7)。因此，Bid转导从细胞内部到线粒体的凋亡信号并促进caspase的激活 (8,9)。

1. Wang, K. et al. (1996) Genes Dev 10, 2859-69.
2. Luo, X. et al. (1998) Cell 94, 481-90.
3. Li, H. et al. (1998) Cell 94, 491-501.
4. Gross, A. et al. (1999) J Biol Chem 274, 1156-63.
5. Li, P. et al. (1997) Cell 91, 479-89.
6. Zou, H. et al. (1999) J Biol Chem 274, 11549-56.
7. Saleh, A. et al. (1999) J Biol Chem 274, 17941-5.
8. Yin, X.M. et al. (1999) Nature 400, 886-91.
9. Korsmeyer, S.J. et al. (2000) Cell Death Differ 7, 1166-73.

原厂资料：

Specificity / Sensitivity

Bid (3C5) Mouse mAb recognizes endogenous levels of total Bid protein. This antibody does not cross-react with endogenous levels of tBid during apoptosis.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the full-length sequence of human Bid protein.

Background

Bid is a pro-apoptotic “BH3 domain-only” member of the Bcl-2 family originally discovered to interact with both the anti-apoptotic family member Bcl-2 and the pro-apoptotic protein Bax (1). Bid is normally localized in the cytosolic fraction of cells as an inactive precursor and is cleaved at Asp60 by caspase-8 during Fas signaling, leading to translocation of the carboxyl terminal p15 fragment (tBid) to the mitochondrial outer membrane (2-4). Translocation of Bid is associated with release of cytochrome c from the mitochondria, leading to complex formation with Apaf-1 and caspase-9 and resulting in caspase-9 activation (5-7). Thus, Bid relays an apoptotic signal from the cell surface to the mitochondria triggering caspase activation (8,9).

1. Wang, K. et al. (1996) Genes Dev 10, 2859-69.
2. Luo, X. et al. (1998) Cell 94, 481-90.
3. Li, H. et al. (1998) Cell 94, 491-501.
4. Gross, A. et al. (1999) J Biol Chem 274, 1156-63.
5. Li, P. et al. (1997) Cell 91, 479-89.
6. Zou, H. et al. (1999) J Biol Chem 274, 11549-56.
7. Saleh, A. et al. (1999) J Biol Chem 274, 17941-5.
8. Yin, X.M. et al. (1999) Nature 400, 886-91.
9. Korsmeyer, S.J. et al. (2000) Cell Death Differ 7, 1166-73.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.