Neurofilament-L (C28E10) Rabbit mAb (Alexa Fluor? 594 Conjugate)

• 产品编号：CST-8743S      品牌：CST       原厂货号：8743S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Homology

Species predicted to react based on 100% sequence homology: Human

Specificity / Sensitivity

Neurofilament-L (C28E10) Rabbit mAb (Alexa Fluor® 594 Conjugate) detects endogenous levels of total Neurofilament-L protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Glu450 of human Neurofilament-L protein.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 594 fluorescent dye and tested in-house for direct immunofluorescent analysis in rat brain. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Neurofilament-L (C28E10) Rabbit mAb #2837.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Human

Specificity / Sensitivity

Neurofilament-L (C28E10) Rabbit mAb (Alexa Fluor® 594 Conjugate) detects endogenous levels of total Neurofilament-L protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Glu450 of human Neurofilament-L protein.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 594 fluorescent dye and tested in-house for direct immunofluorescent analysis in rat brain. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Neurofilament-L (C28E10) Rabbit mAb #2837.

Background

The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (2). While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3,4). Research studies have shown neurofilament accumulations in many human neurological disorders including Parkinson's disease (in Lewy bodies along with α-synuclein), Alzheimer's disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS) (1).

1. Al-Chalabi, A. and Miller, C.C. (2003) Bioessays 25, 346-355.
2. Cohlberg, J.A. et al. (1995) J. Biol. Chem. 270, 9334-9339.
3. Hisanaga, S. et al. (1994) Mol. Biol. Cell 5, 161-172.
4. Sihag, R.K. et al. (1999) J. Neurochem. 72, 491-499.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4ºC. Protect from light. Do not
freeze.