Phospho-CK2 Substrate [(pS/pT)DXE] MultiMab? Rabbit mAb mix

• 产品编号：CST-8738S      品牌：CST       原厂货号：8738S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-(Ser/Thr) CK2 Substrate (P-S/T3-100) Rabbit mAb兔单抗能够检测内源性的含有CK2 磷酸化共有序列pS/pTDXE 基序的蛋白。该抗体是研究CK2底物的有力工具。该单克隆抗体是经由合成的含有T*DXE基序的多肽免疫动物制备。Casein Kinase II (CK2)是一个高度保守、广泛表达，具有组成型活性的四聚体Ser/Thr 蛋白激酶，具有多种参与细胞周期进程、凋亡、转录、炎症以及DNA损伤应激等一系列细胞进程调控的底物。研究发现，CK2与病毒感染、癌症及其他疾病相关（1-5）。CK2底物包含多种酸性残基(Asp和 Glu)，位于磷酸化Ser或Thr残基的下游。CK2底物的共有序列是pS/pTD/EXD/E，最关键的是+3 位的氨基酸残基，之后是+1 位的氨基酸残基（6）。

原厂资料：

Specificity / Sensitivity

Phospho-(Ser/Thr) CK2 Substrate (P-S/T3-100) Rabbit mAb recognizes endogenous proteins containing a pS/pTDXE motif, which is a CK2 phosphorylation consensus sequence. This antibody is a useful tool to study CK2 substrates.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the T*DXE motif.

Background

Casein Kinase II (CK2) is a highly conserved, ubiquitously expressed, and constitutively active tetrameric Ser/Thr protein kinase with hundreds of substrates participating in the regulation of a variety of cellular processes including cell cycle progression, apoptosis, transcription, inflammation, and the DNA damage response. Research studies have implicated CK2 in roles related to viral infection, cancer, and other diseases (1-5). CK2 substrates contain multiple acidic residues (Asp and Glu) located downstream of the phosphorylated Ser or Thr residue. The consensus sequence for CK2 substrates is pS/pTD/EXD/E with the most crucial residue at the +3 position followed by the residue at the +1 position (6).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.