RXRβ Antibody

• 产品编号：CST-8715S      品牌：CST       原厂货号：8715S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

RXRβ Antibody识别内源性水平的总RXRβ蛋白。该抗体不与RXRα或RXRγ蛋白中的任何一个发生交叉反应。该多克隆抗体通过用合成肽免疫动物制备，该合成肽是人RXRβ蛋白靠近氨基末端的残基。抗体由蛋白A和肽亲和层析纯化。人视黄醇X受体（RXRs）由三种不同的基因（RXRα，RXRβ，RXRγ）编码，并与具有高亲和力的维生素A衍生物，9-顺-视黄酸选择性结合。 RXRs是II型核激素受体，主要位于核室，非依赖性配体结合。核RXR与核激素受体亚家族蛋白形成异二聚体，包括甲状腺激素受体、维甲酸受体、维生素D受体、过氧化物酶体增殖物激活受体、肝脏X受体、法尼酯X受体（1）。由于RXR与多个核激素受体形成异源二聚体，通过与靶基因启动子/增强区域的顺式作用元件结合，在众多激素信号转导通路的转录调控中发挥核心作用（2）。RXRβ，像RXR超家族其他成员一样，有一个典型的三部分模块化结构，包括：（a）一个高度保守的中央区域，含有C4/C5锌指结构域，负责DNA结合；（b）一个相对高度保守的C-末端区域，其中包含激素结合域和二聚体化功能域；及（c）一个可变的N末端结构域，与靶基因的反式激活或抑制有关（2）。N末端结构域的可变性被认为是选择性剪接和/或差动启动子使用的结果（3-5）。由于它能够结合小鼠的主要组织相容性复合体（MHC）I类启动子的调节区II，也简称为H-2RIIBP，小鼠RXRβ首先被识别(6)。在子宫内，遗传切除Rxrb的小鼠会有50％致死，在存活的雄性小鼠中，对精子有影响从而导致不育（7）。进一步研究表明，用表达含受损AF-2核的Rxrb突变体导致塞尔托利细胞中脂质代谢异常，提示Rxrb和其他核受体之间的功能性相互作用，控制血脂代谢（8）。

1. Gronemeyer, H. et al. (2004) Nat Rev Drug Discov 3, 950-64.
2. Mangelsdorf, D.J. et al. (1992) Genes Dev 6, 329-44.
3. Nagata, T. et al. (1994) Gene 142, 183-9.
4. Fleischhauer, K. et al. (1993) Hum Genet 90, 505-10.
5. Fleischhauer, K. et al. (1992) Nucleic Acids Res 20, 1801.
6. Hamada, K. et al. (1989) Proc Natl Acad Sci USA 86, 8289-93.
7. Kastner, P. et al. (1996) Genes Dev 10, 80-92.
8. Mascrez, B. et al. (2004) EMBO Rep 5, 285-90.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat, Monkey, Bovine, Dog, Pig

Specificity / Sensitivity

RXRβ Antibody recognizes endogenous levels of total RXRβ protein. This antibody does not cross-react with either RXRα or RXRγ proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human RXRβ protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The human retinoid X receptors (RXRs) are encoded by three distinct genes (RXRα, RXRβ, and RXRγ) and bind selectively and with high affinity to the vitamin A derivative, 9-cis-retinoic acid. RXRs are type-II nuclear hormone receptors that are largely localized to the nuclear compartment independent of ligand binding. Nuclear RXRs form heterodimers with nuclear hormone receptor subfamily 1 proteins, including thyroid hormone receptor, retinoic acid receptors, vitamin D receptor, peroxisome proliferator-activated receptors, liver X receptors, and farnesoid X receptor (1). Since RXRs heterodimerize with multiple nuclear hormone receptors, they play a central role in transcriptional control of numerous hormonal signaling pathways by binding to cis-acting response elements in the promoter/enhancer region of target genes (2).RXRβ, like other members of the RXR subfamily, possesses a characteristic tripartite modular structure consisting of (a) a highly conserved central region containing the C4/C5 zinc-finger domain, which is responsible for DNA binding; (b) a relatively well-conserved C-terminal region, which contains the hormone binding and dimerization domains; and (c) a variable N-terminal domain, which has been implicated in either transactivation or repression of target genes (2). Variability within the N-terminal domain is thought to be the result of alternative splicing and/or differential promoter usage (3-5). The murine RXRβ was initially identified because of its ability to bind to the regulatory region II in the murine major histocompatability complex (MHC) class I promoter and is therefore also referred to as H-2RIIBP (6). Genetic ablation of murine Rxrb produced approximately 50% lethality in utero and males that survived had defects of spermatazoa, which resulted in sterility (7). Further studies revealed that expression of a Rxrb mutant with an impaired AF-2 core led to abnormal lipid metabolism in Sertoli cells, suggesting functional interactions between Rxrb and other nuclear receptors that control lipid metabolism (8)

1. Gronemeyer, H. et al. (2004) Nat Rev Drug Discov 3, 950-64.
2. Mangelsdorf, D.J. et al. (1992) Genes Dev 6, 329-44.
3. Nagata, T. et al. (1994) Gene 142, 183-9.
4. Fleischhauer, K. et al. (1993) Hum Genet 90, 505-10.
5. Fleischhauer, K. et al. (1992) Nucleic Acids Res 20, 1801.
6. Hamada, K. et al. (1989) Proc Natl Acad Sci USA 86, 8289-93.
7. Kastner, P. et al. (1996) Genes Dev 10, 80-92.
8. Mascrez, B. et al. (2004) EMBO Rep 5, 285-90.

注意事项：

Storage: Supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl,
100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot
the antibody