PRK2 (D10A10) Rabbit mAb

• 产品编号：CST-8697S      品牌：CST       原厂货号：8697S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PRK2 (D10A10) Rabbit mAb识别内源性水平总PRK2蛋白水平。该抗体不与PRK1发生交叉反应。该单克隆抗体通过用合成肽免疫动物制备，该合成肽与人PRK2蛋白亮氨酸（633位）附近的残基一样。蛋白激酶C相关激酶（PRKs）是一种丝氨酸/苏氨酸特异性激酶亚家族，具有与PKC家族具有高度同源性的催化结构域（1-3）。它们是Rho家族GTP酶的效应物（4-6），在体外由脂肪酸和磷脂激活（7,8）。体外和体内的激活涉及PDK1磷酸化修饰PRK1 (Thr774)/PRK2 (Thr816)的活性环（9,10）。

1. Mukai, H. et al. (1994) Biochem. Biophys. Res. Commun. 199, 897-904.
2. Morrice, N.A. et al. (1994) J. Biol. Chem. 269, 20040-20046.
3. Palmer, R.H. et al. (1994) FEBS Lett. 356, 5-8.
4. Watanabe, G. et al. (1996) Science 271, 645-648.
5. Amano, M. et al. (1996) Science 271, 648-650.
6. Vincent, S. and Settleman, J. (1997) Mol. Cell. Biol. 17, 2247-2256.
7. Morrice, N.A. et al. (1994) FEBS Lett. 351, 171-175.
8. Palmer, R.H. et al. (1995) J. Biol. Chem. 270, 22412-22416.
9. Flynn, P. et al. (2000) J. Biol. Chem. 275, 11064-70.
10. Dong, L.Q. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 5089-94.

原厂资料：

Specificity / Sensitivity

PRK2 (D10A10) Rabbit mAb recognizes endogenous levels of total PRK2 protein. This antibody does not cross-react with PRK1.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu633 of human PRK2 protein.

Background

The protein kinase C-related kinases (PRKs) are a subfamily of Ser/Thr-specific kinases with a catalytic domain highly homologous to the PKC family (1-3). They are effectors of Rho family GTPases (4-6) and are activated by fatty acids and phospholipids in vitro (7,8). Activation in vitro and in vivo involves the activation loop phosphorylation of PRK1 (Thr774)/PRK2 (Thr816) by PDK1 (9,10).

1. Mukai, H. et al. (1994) Biochem. Biophys. Res. Commun. 199, 897-904.
2. Morrice, N.A. et al. (1994) J. Biol. Chem. 269, 20040-20046.
3. Palmer, R.H. et al. (1994) FEBS Lett. 356, 5-8.
4. Watanabe, G. et al. (1996) Science 271, 645-648.
5. Amano, M. et al. (1996) Science 271, 648-650.
6. Vincent, S. and Settleman, J. (1997) Mol. Cell. Biol. 17, 2247-2256.
7. Morrice, N.A. et al. (1994) FEBS Lett. 351, 171-175.
8. Palmer, R.H. et al. (1995) J. Biol. Chem. 270, 22412-22416.
9. Flynn, P. et al. (2000) J. Biol. Chem. 275, 11064-70.
10. Dong, L.Q. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 5089-94.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.