Lysine Methyltransferase Antibody Sampler Kit

产品编号：CST-8694S 品牌：Cell Signaling Technology 原厂货号：8694S
产品分类：抗体 > 一抗 > 复合抗体试剂盒
应用分类：

包装：

1Kit

运保温度:

–20°C

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其它组分：

RBBP5 (D3I6P) Rabbit mAb 【子货号：#13171，包装：40 µl，，运保温度：–20°C】

ESET (C1C12) Rabbit mAb 【子货号：#2196，包装：40 µl，，运保温度：–20°C】

SET7/SET9 Antibody【子货号： #2813，包装：40 µl，，运保温度：–20°C】

SET8 (C18B7) Rabbit mAb 【子货号：#2996，包装：40 µl，，运保温度：–20°C】

G9a/EHMT2 (C6H3) Rabbit mAb 【子货号：#3306，包装：40 µl，，运保温度：–20°C】

ASH2L (D93F6) XP® Rabbit mAb 【子货号：#5019，包装：40 µl，，运保温度：–20°C】

Anti-rabbit IgG, HRP-linked Antibody 【子货号：#7074，包装：40 µl，，运保温度：–20°C】

SUV39H1 (D11B6) Rabbit mAb 【子货号：#8729，包装：40 µl，，运保温度：–20°C】

SMYD2 (D14H7) Rabbit mAb 【子货号：#9734，包装：40 µl，，运保温度：–20°C】

描述：

试剂盒中的每种抗体仅识别特异的目的蛋白且不会与其他家族成员产生交叉反应。单克隆抗体是由合成的多肽免疫动物而生产的，对应序列信息如下：人ASH2L蛋白，人ESET蛋白的羧基端，人G9a/EHMT2蛋白的羧基端，人SET8蛋白，围绕人SMYD2蛋白Val414的残基或围绕人SUV39H1 Asp380的残基。多克隆抗体是经由合成的围绕人RBBP5蛋白的Val407残基或人SET7/SET9蛋白的多肽免疫动物而生产的。多克隆抗体由蛋白质A和多肽亲和层析纯化而成。Lysine Methyltransferase Antibody Sampler Kit可以提供一种快速而经济的方法来检测内源性赖氨酸甲基转移酶的水平。试剂盒提供的每种一抗都足够进行4次western blot实验。含SET结构域的蛋白是一类潜在的组蛋白甲基转移酶(HMTase)，根据他们假定的底物专一性可分成不同的亚类。组蛋白H3 Lys9位点 (H3-K9) 甲基转移酶组基因包括Suv39h1、Suv39h2、G9a、G9a相关蛋白(GLP)和SETDB1/ESET (1)。H3-K9 位点的甲基化标记是含chromo蛋白的结合位点，扮演着重要角色，能够导致染色质凝集和异染色质的形成(2)。组蛋白H3-K4甲基化仅与转录活跃的基因有关(2)。第一个H3-K4甲基化酶复合物，是在酿酒酵母中发现的COMPASS。其由Set1/KMT2和另外七种多肽， Cps60-Cps15 (2)组成。Set1/KMT2在COMPASS内发挥功能，可单、二和三甲基化 H3-K4 (2)位点。在哺乳动物也存在一些Set1相关蛋白，包括WDR5、RBBP5、ASH2L、CXXC1和DPY30 (2,3)。SET7/SET9是含SET结构域蛋白家族的成员之一，能特异性的甲基化 H3-K4位点、TAF10 Lys189位点，TFIID转录因子复合物的成员，以及p53肿瘤抑制蛋白的Lys372位点。含SET结构域的赖氨酸甲基转移酶 8 (SET8)，亦称含PR/SET结构域蛋白7 (PR/SET7)，只含有一个亚基，能够将组蛋白H4-K20位点单甲基化，且底物为核小体时更好(7-9)。含SET和MYND结构域蛋白2 (SMYD2)，亦称赖氨酸甲基转移酶蛋白3C (KMT3C)，能够与Sin3A阻遏复合物相互作用并且将H3-K36位点甲基化，从而阻遏转录 (10)。SMYD2还能通过与HSP90α的相互作用来甲基化H3-K4位点，并且能够甲基化p53 Lys370 位点从而阻遏p53介导的转录激活和细胞凋亡(11,12)。

原厂资料：

Specificity / Sensitivity

Each antibody in this kit recognizes only the specific target protein and does not cross-react with other family members.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human ASH2L protein, the carboxy terminus of human ESET protein, the carboxy terminus of human G9a/EHMT2 protein, human SET8 protein, residues surrounding Val414 of human SMYD2 protein, or residues surrounding Asp380 of human SUV39H1 protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val407 of human RBBP5 protein or the human SET7/SET9 protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Description

The Lysine Methyltransferase Antibody Sampler Kit provides a fast and economical means to evaluate endogenous levels of lysine methyltransferases. The kit contains enough primary antibody to perform four western blot experiments per primary antibody.

Background

SET domain-containing proteins are potential histone methyltransferases (HMTases), which are classified into subgroups by their putative substrate specificities. Histone H3 Lys9 (H3-K9) methyltransferase group genes include Suv39h1, Suv39h2, G9a, G9a related protein (GLP) and SETDB1/ESET (1). The H3-K9 methylation mark plays an important role as a binding site for the chromo-containing protein, resulting in chromatin compaction and heterochromatin generation (2). Histone H3-K4 methylation is exclusively associated with actively transcribed genes (2). The first H3-K4 methylase complex, COMPASS, was identified in the yeast S. cerevisiae and consists of Set1/KMT2 and seven other polypeptides, Cps60-Cps15 (2). Set1/KMT2 functions within COMPASS and is capable of mono-, di-, and trimethylating H3-K4 (2). There are several Set1 related proteins in mammals including WDR5, RBBP5, ASH2L, CXXC1, and DPY30 (2,3). SET7/SET9 is a member of the SET domain-containing family that can specifically methylate H3-K4, Lys189 of the TAF10, a member of the TFIID transcription factor complex, and Lys372 of the p53 tumor suppressor protein (4-6). SET domain-containing lysine methyltransferase 8 (SET8), also known as PR/SET domain-containing protein 7 (PR/SET7), is a single-subunit enzyme that mono-methylates histone H4-K20, preferably on nucleosomal substrates (7-9). SET and MYND domain-containing protein 2 (SMYD2), also known as lysine methyltransferase protein 3C (KMT3C), functions to repress transcription by interacting with the Sin3A repressor complex and methylating H3-K36 (10). SMYD2 also methylates H3-K4 through interaction with HSP90α, and methylates p53 at Lys370 to repress p53-mediated transcriptional activation and apoptosis (11,12).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibodies.