CtBP1 (D2D6) Rabbit mAb

• 产品编号：CST-8684S      品牌：CST       原厂货号：8684S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CtBP1 (D2D6) Rabbit mAb兔单抗识别内源性的总CtBP1蛋白。单克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与邻近人源CtBP1蛋白的氨基末端氨基酸序列一致。CtBP1(C-末端结合蛋白1)被认为是一种与腺病毒E1A D的C末端蛋白相互作用的细胞因子。腺病毒E1A D的C末端蛋白涉及细胞内一些关键基因的转录调控[1]。在发育过程中CtBP1能通过与多梳组合蛋白（PcG）相互作用[4]，和其固有的脱氢酶活性[2,3]调控基因活性。连同它的同源物CtBP2，一种锌指同源异构域deltaEF1的转录共抑制因子， 通过转录抑制机制调控一大类细胞内反应进程[5]。通过与PRDM16的直接相互作用，CtBP1调节白色脂肪基因抑制和直接向棕色脂肪基因程序的分化来调控棕色脂肪组织的分化[6]， CtBP1也通过调节操控高尔基体管状网络的分裂机制来对脂肪代谢和膜分裂起作用[7]。CtBP1最近被报道通过氧化还原机制来抑制BRCA1的转录[8]。而且，在侵袭性导管乳腺癌中下调的BRCA1和E- cadherin被认为和CtBP1激活正相关[9]。

1. Schaeper, U. et al. (1995) Proc Natl Acad Sci USA 92, 10467-71.
2. Balasubramanian, P. et al. (2003) FEBS Lett 537, 157-60.
3. Kumar, V. et al. (2002) Mol Cell 10, 857-69.
4. Sewalt, R.G. et al. (1999) Mol Cell Biol 19, 777-87.
5. Furusawa, T. et al. (1999) Mol Cell Biol 19, 8581-90.
6. Kajimura, S. et al. (2008) Genes Dev 22, 1397-409.
7. Cassens, U. et al. (1999) Transfus Med 9, 311-20.
8. Deng, Y. et al. (2010) Oncogene 29, 6603-8.
9. Deng, Y. et al. (2011) Mol Carcinog, Epub ahead of print.

原厂资料：

Specificity / Sensitivity

CtBP1 (D2D6) Rabbit mAb recognizes endogenous levels of total CtBP1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human CtBP1 protein.

Background

CtBP1 (C-terminal binding protein 1) was first recognized as a cellular factor that interacts with the C-terminal portion of adenovirus E1A, a protein involved in the transcriptional regulation of key cellular genes (1). CtBP1 is able to regulate gene activity through its intrinsic dehydrogenase activity (2,3) and by interacting with Polycomb Group (PcG) proteins during development (4). Along with its homologue, CtBP2, it acts as a transcriptional corepressor of zinc-finger homeodomain factor deltaEF1 to regulate a wide range of cellular processes through transrepression mechanisms (5). Through its direct interaction with PRDM16, CtBP1 has been shown to be involved in brown adipose tissue differentiation by mediating the repression of white fat genes and directing differentiation toward the brown fat gene program (6). CtBP1 also plays a role in lipid metabolic pathways and membrane fission by regulating the fission machinery operating Golgi tubular networks (7). CtBP1 has recently been shown to repress transcription of BRCA1 via a redox regulated mechanism (8). Furthermore, it is thought that downregulation of BRCA1 and E-cadherin in invasive ductal breast carcinoma correlates directly with activation of CtBP1 (9).

1. Schaeper, U. et al. (1995) Proc Natl Acad Sci USA 92, 10467-71.
2. Balasubramanian, P. et al. (2003) FEBS Lett 537, 157-60.
3. Kumar, V. et al. (2002) Mol Cell 10, 857-69.
4. Sewalt, R.G. et al. (1999) Mol Cell Biol 19, 777-87.
5. Furusawa, T. et al. (1999) Mol Cell Biol 19, 8581-90.
6. Kajimura, S. et al. (2008) Genes Dev 22, 1397-409.
7. Cassens, U. et al. (1999) Transfus Med 9, 311-20.
8. Deng, Y. et al. (2010) Oncogene 29, 6603-8.
9. Deng, Y. et al. (2011) Mol Carcinog, Epub ahead of print.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium
azide. Store at –20°C. Do not aliquot the antibody