Tight Junction Antibody Sampler Kit

• 产品编号：CST-8683S      品牌：Cell Signaling Technology       原厂货号：8683S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

CD2AP抗体识别内源性的CD2AP蛋白。Claudin-1抗体识别内源性的总claudin-1蛋白。基于序列的相似性，Claudin-1抗体可能可以与claudin-2蛋白进行交叉反应。Occludin (D15G7)兔源单克隆抗体识别内源性的总occludin蛋白。ZO-1 (D7D12)兔源单克隆抗体识别内源性的总ZO-1 蛋白。ZO-2抗体识别内源性的总ZO-2蛋白。ZO-3 (D57G7) XP® 兔源单克隆抗体识别内源性的总ZO-3 蛋白。多克隆抗体由人CD2AP蛋白、鼠claudin-1蛋白或鼠ZO-2蛋白碳-末端附近的残基合成多肽免疫动物产生。该抗体由protein A和肽亲和层析纯化。单克隆抗体由人occludin蛋白或人ZO-1或人ZO-3蛋白碳-末端附近的残基合成多肽免疫动物产生The Tight Junction Antibody Sampler Kit 提供了经济的方法来评估在紧密连接中的许多蛋白。该试剂盒包括了充足的一抗，每个一抗可以进行4次western blot试验。紧密连接，或闭锁小带，横越上皮细胞核内皮细胞对液体形成了连续的屏障。他们对于细胞周边的渗透性、维持细胞极性、阻止细胞尖部和细胞底部表面的跨膜蛋白的移动起调节作用(reviewed in 1)。紧密连接由claudin 和occludin跨膜蛋白组成，这两者将连接联接到细胞骨架(1,2)。Occludin被认为对紧密连接的组成和维持起重要作用。不同位点的Occludin磷酸化可能调节与其他紧密连接蛋白的相互作用例如ZO-1 (3)。闭锁小带蛋白ZO-1, -2, 和-3(又被称为 TJP1, 2, 和3)是膜周围调节蛋白，连接紧密连接的跨膜蛋白例如occludin 和claudin到肌动细胞骨架(reviewed in 4)。ZO-1和 ZO-2对于紧密连接的形成和功能都是必需的(5,6)。在亚融合状态的增殖细胞中，ZO-1和 ZO-2已经被证实共同定位于细胞核，并在转录调节中起作用(7-9)。ZO-3氨基末端部分外源性的表达赋予对干扰紧密连接及粘附连接组装的显性负效应(10)。CD2AP是一种鹰架蛋白，可以连接膜蛋白与细胞骨架(11-13)。CD2AP在特殊细胞类型如肾小球的裂孔隔膜的紧密连接的形成中起重要作用(14)。CD2AP也与CD2-表达T细胞核抗原提呈细胞的免疫突触相关(15)。CD2AP与其他细胞骨架蛋白的相互作用有可能调节EGFR的胞吞作用(13)。

原厂资料：

Specificity / Sensitivity

CD2AP Antibody recognizes endogenous levels of total CD2AP protein. Claudin-1 Antibody recognizes endogenous levels of total claudin-1 protein. Based on sequence similarity, Claudin-1 Antibody may cross-react with claudin-2 protein. Occludin (D15G7) Rabbit mAb recognizes endogenous levels of total occludin protein. ZO-1 (D7D12) Rabbit mAb recognizes endogenous levels of total ZO-1 protein. ZO-2 Antibody recognizes endogenous levels of total ZO-2 protein. ZO-3 (D57G7) XP® Rabbit mAb detects endogenous levels of total ZO-3 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues near the carboxy terminus of human CD2AP protein, mouse claudin-1 protein, or mouse ZO-2 protein. Antibodies are purified using protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to central residues of human occludin protein or residues near the carboxy terminus of human ZO-1 protein or human ZO-3 protein.

Description

The Tight Junction Antibody Sampler Kit provides an economical means to evaluate the presence of a number of proteins involved in tight junctions. The kit contains enough primary antibodies to perform four western blot experiments per primary antibody.

Background

Tight junctions, or zona occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and basolateral cell surfaces (reviewed in 1). Tight junctions are composed of claudin and occludin transmembrane proteins, which join the junctions to the cytoskeleton (1,2). Occludin is thought to be important in the assembly and maintenance of tight junctions. Differential phosphorylation of occludin at various residues may regulate its interaction with other tight junction proteins such as ZO-1 (3). Zona occludens proteins ZO-1, -2, and -3 (also known as TJP1, 2, and 3) are peripheral membrane adaptor proteins that link junctional transmembrane proteins such as occludin and claudin to the actin cytoskeleton (reviewed in 4). ZO-1 and ZO-2 are required for tight junction formation and function (5,6). In subconfluent proliferating cells, ZO-1 and ZO-2 have been shown to colocalize to the nucleus and play a role in transcriptional regulation (7-9). Exogenous expression of the amino terminal portion of ZO-3 exerts a dominant negative effect that interferes with assembly of tight junctions and adherens junctions (10). CD2AP is a scaffolding protein that is thought to link membrane proteins to the cytoskeleton (11-13). It plays a role in formation of tight junctions in specialized cell types such as the slit diaphragm in the kidney glomerulus (14). CD2AP is also involved in the immunological synapse between CD2-expressing T cells and antigen presenting cells (15). It has been shown that interaction between CD2AP and other cytoskeletal proteins may regulate the endocytosis of EGFR (13).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibodies.