Importin β1 Antibody

• 产品编号：CST-8673S      品牌：Cell Signaling Technology       原厂货号：8673S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Importin β1抗体可以识别内源性总的Importin β1蛋白。多抗由合成肽段免疫动物产生，该肽段与人importin β1蛋白Pro307残基邻近序列一致。抗体由蛋白A和肽段亲和层析技术纯化得到。mportins属于karyopherin核转运蛋白家族（1），分成两个亚家族：importin α和importin β。Importins主要在核蛋白的输入和输出过程中发挥作用（2,3）。Importin β1（也被称为karyopherin β1, Kpnβ1, Kpnb1, 或p97）在细胞核输入过程发挥了关键作用（1-3）。细胞核通过importin β1与接头importin α（也称为karyopherin α, 或 Kpnα）进行输入过程，是经典核定位信号(NLS)通路的重要组分（4）。Importin α可以直接识别cargo taget的NLS，促进与importin β1形成复合物。cargo:importin α:importin β1复合物通过核孔复合物（NPC）转运入核，该复合物可以被RanGTP解离（1-4）。通过importin β1的入核作用也存在于cargo 蛋白的importin β1识别，绕过importin α的过程中。在两种情况下，importinβ1/靶蛋白相互作用都是通过importin β1 HEAT重复区域靶蛋白（cargo蛋白或importin α）序列相互结合发生的(5)。

1. Chook, Y.M. and Blobel, G. (2001) Curr Opin Struct Biol 11, 703-15.
2. Pemberton, L.F. and Paschal, B.M. (2005) Traffic 6, 187-98.
3. Moroianu, J. (1998) J Cell Biochem 70, 231-9.
4. Lange, A. et al. (2007) J Biol Chem 282, 5101-5.
5. Marfori, M. et al. (2011) Biochim Biophys Acta 1813, 1562-77.

原厂资料：

Specificity / Sensitivity

Importin β1 Antibody detects endogeneous levels of total importin β1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro307 of human importin β1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Importins belong to the karyopherin family of nuclear transport proteins (1) and are divided into two subgroups: importin α and importin β. Importins mainly function in nuclear protein import and export (2,3). Importin β1 (also known as karyopherin β1, Kpnβ1, Kpnb1, or p97) plays a key role in the nuclear import process (1-3). Nuclear import via importin β1 association with adaptor importin α (also known as karyopherin α, or Kpnα) is an essential component of the classical nuclear localization signal (NLS) pathway (4). Importin α directly recognizes the NLS present in the cargo taget, prompting complex formation with importin β1. The cargo:importin α:importin β1 complex is transported across the nuclear pore complex (NPC) into the nucleus, where it is dissociated by the binding of RanGTP (1-4). Nuclear import directly via importin β1 can also occur by importin β1 recognition of the cargo protein, bypassing importin α involvement. In both cases, the importin β1/target protein interaction is mediated through the binding of importin β1 HEAT repeats with the target protein sequences (either the cargo protein itself or importin α) (5).

1. Chook, Y.M. and Blobel, G. (2001) Curr Opin Struct Biol 11, 703-15.
2. Pemberton, L.F. and Paschal, B.M. (2005) Traffic 6, 187-98.
3. Moroianu, J. (1998) J Cell Biochem 70, 231-9.
4. Lange, A. et al. (2007) J Biol Chem 282, 5101-5.
5. Marfori, M. et al. (2011) Biochim Biophys Acta 1813, 1562-77.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.