Organelle Localization IF Antibody Sampler Kit

产品编号：CST-8653S 品牌：Cell Signaling Technology 原厂货号：8653S
产品分类：抗体 > 一抗 > 复合抗体试剂盒
应用分类：

包装：

1Kit

运保温度:

–20°C

到货周期：

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其它组分：

RCAS1 (D2B6N) XP® Rabbit mAb 【子货号：#12290，包装：40 µl，，运保温度：–20°C】

EEA1 (C45B10) Rabbit mAb 【子货号：#3288，包装：40 µl，，运保温度：–20°C】

PDI (C81H6) Rabbit mAb 【子货号：#3501，包装：40 µl，，运保温度：–20°C】

AIF (D39D2) XP® Rabbit mAb 【子货号：#5318，包装：40 µl，，运保温度：–20°C】

LAMP1 (D2D11) XP® Rabbit mAb 【子货号：#9091，包装：40 µl，，运保温度：–20°C】

描述：

Organelle Localization IF Antibody Sampler Kit中的每个抗体仅识别其特异的靶点，和其他家族成员没有交叉反应。每个抗体都经IF-IC验证并根据上述方法进行细胞器染色。这些蛋白的表达在不同细胞和组织中可能有所不同。请参阅www.cellsignal.com，以获得试剂盒中各个组成部分的另外的专一性/敏感性的信息。兔单克隆抗体通过用人LAMP1蛋白的重组蛋白片段或合成肽免疫动物制备，这些合成肽是人AIF蛋白Ala520周围的残基，人EEA1蛋白Ser70周围的残基，人PDI蛋白的序列或人RCAS1蛋白Gly147周围的残基。Organelle Localization IF Antibody Sampler Kit通过免疫细胞化学荧光法（IF-IC）提供了一种经济的手段来鉴定细胞器。本试剂盒包含的一抗至少能进行20次IF-IC试验或每种抗体至少能进行4次western blot。蛋白质的亚细胞定位知识可能揭示其潜在的在多种细胞过程中的作用。我们可以根据亚细胞位置的标记与本试剂盒中细胞器特异的抗体处于相同位置来进行亚细胞定位。这些抗体可以作为免疫荧光的有力工具，也可以用作分级分离细胞裂解物的免疫印迹对照。

原厂资料：

Specificity / Sensitivity

Each antibody in the Organelle Localization IF Antibody Sampler Kit recognizes only its specific target and does not cross-react with other family members. Each antibody has been validated for IF-IC and stains the organelles indicated above. Expression of these proteins may vary in different cells and tissues. Please see www.cellsignal.com for additional specificity/sensitivity information for individual kit components.

Source / Purification

Rabbit monoclonal antibodies are produced by immunizing animals with a recombinant protein fragment of human LAMP1 protein, or with a synthetic peptide corresponding to residues surrounding Ala520 of human AIF protein, residues surrounding Ser70 of human EEA1 protein, the sequence of human PDI protein, or residues surrounding Gly147 of human RCAS1 protein.

Description

The Organelle Localization IF Antibody Sampler Kit provides an economical means for identification of cellular organelles by fluorescence immunocytochemistry (IF-IC). This kit includes enough primary antibody to perform at least twenty IF-IC tests or four western blots with each antibody.

Background

Knowledge of the subcellular location of a protein may reveal its potential role in a variety of cellular processes. One can confirm the subcellular location of a marker that colocalizes with one of the organelle-specific antibodies in this kit. While these antibodies serve as powerful tools for immunofluorescence, they may also be used as western blot controls for fractionated cell lysates.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibodies