BATF (D7C5) Rabbit mAb

• 产品编号：CST-8638S      品牌：CST       原厂货号：8638S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

BATF (D7C5) Rabbit mAb能够检测内源BATF蛋白总体水平。此单克隆抗体是通过合成人源对应的 BATF C-末端周围的肽段来免疫动物而获得。碱性亮氨酸拉链转录因子ATF-like (BATF) 是碱性亮氨酸拉链(bZIP) 转录因子，并且是AP-1/ATF 家族成员，它能够与 Jun家族的成员形成抑制性的二聚体(1-3)。BATF的高表达主要限制在成熟的T细胞中, 在B细胞中的表达被包括病毒感染在内的免疫应答所诱导(1,2)。BATF的表达也可通过Stat3依赖而被IL-6 诱导(4)。BATF在免疫细胞谱系的分化过程中起了重要的作用(5-7)。研究BATF缺失的小鼠表明BATF在形成表达IL-17的Th17细胞过程中发挥了重要的作用, 部分原因是通过调控IL-17的表达(5,6)。BATF敲除小鼠对实验中自体免疫脑脊髓炎(EEA)有抵抗作用, 这与自体免疫模型中对Th17细胞的作用是相吻合的(5)。另外的研究发现BATF对产生抗体类别的转换有重要的作用。BATF对产生卵泡辅助T细胞(Tfh)是必需的, 而且是通过调控BCL6和 c-Maf (6,7)。在B细胞中, BATF对激活诱导胞啶脱氨基酶(AID)的表达和抗体类别转换的响应有调控作用(7)。综上所述，这样研究暗示了BATF对不同类群的免疫细胞是重要的调控因子。

1. Dorsey, M.J. et al. (1995) Oncogene 11, 2255-65.
2. Hasegawa, H. et al. (1996) Biochem Biophys Res Commun 222, 164-70.
3. Echlin, D.R. et al. (2000) Oncogene 19, 1752-63.
4. Senga, T. et al. (2002) Oncogene 21, 8186-91.
5. Schraml, B.U. et al. (2009) Nature 460, 405-9.
6. Betz, B.C. et al. (2010) J Exp Med 207, 933-42.
7. Ise, W. et al. (2011) Nat Immunol 12, 536-43.

原厂资料：

Specificity / Sensitivity

BATF (D7C5) Rabbit mAb detects endogenous levels of total BATF protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human BATF protein.

Background

Basic leucine zipper transcriptional factor ATF-like (BATF) is a basic leucine zipper (bZIP) transcription factor and is part of the AP-1/ATF family that forms inhibitory dimers with members of the Jun family (1-3). Expression of BATF is largely restricted with highest levels found in mature T cells, and it is induced in B cells following immune responses including viral infection (1,2). BATF expression is also induced by IL-6 via a Stat3-dependent mechanism (4). BATF plays an important role in the differentiation of immune cell lineages (5-7). Studies of BATF-deficient mice have demonstrated a critical role for BATF in the formation of IL-17-expressing Th17 cells, in part, by regulating the expression of IL-17 (5,6). BATF knockouts are resistant to experimental autoimmune encephalomyelitis (EEA), consistent with the role of Th17 cells in this model for autoimmunity (5). Additional studies have found that BATF is important in generating antibody class switching. BATF is required for the generation of follicular helper T cells (Tfh), by regulating BCL6 and c-Maf (6,7). In B cells, BATF controls the expression of activation-induced cytidine deaminase (AID) and regulates class-switched antibody responses (7). Taken together, these studies suggest that BATF is a key regulator of distinct populations of immune cells.

1. Dorsey, M.J. et al. (1995) Oncogene 11, 2255-65.
2. Hasegawa, H. et al. (1996) Biochem Biophys Res Commun 222, 164-70.
3. Echlin, D.R. et al. (2000) Oncogene 19, 1752-63.
4. Senga, T. et al. (2002) Oncogene 21, 8186-91.
5. Schraml, B.U. et al. (2009) Nature 460, 405-9.
6. Betz, B.C. et al. (2010) J Exp Med 207, 933-42.
7. Ise, W. et al. (2011) Nat Immunol 12, 536-43.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody