LASP1 Antibody

• 产品编号：CST-8636S      品牌：CST       原厂货号：8636S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

LASP1抗体可以识别内源性总的LASP1蛋白。多抗由合成肽段免疫动物产生，该肽段与人LASP1蛋白Ala191残基邻近序列一致。抗体由蛋白A和肽段亲和层析技术纯化得到。LASP1是一个细胞骨架折叠蛋白属于LIM蛋白超家族（1,2）。LASP1由一个N-末端LIM结构域，随后的2个伴肌动蛋白重复，和C-末端SH3结构域组成（1,3）。伴肌动蛋白重复与actin结合，SH3结构域与palladin结合（4,5），提示LASP1作为actin结合蛋白发挥功能，可能残余了细胞骨架重构。研究显示LASP1定位在黏着斑，板状伪足和膜褶皱位置（6-8）可能涉及了膜的迁移。LASP1的过表达与转移癌相关，例如乳腺癌和卵巢癌（2）。在这些情况下，肿瘤细胞的细胞膜，细胞质和细胞核中都有报道发现LASP1，说明LASP1涉及到了细胞膜和细胞核通路（9,10）。

1. Tomasetto, C. et al. (1995) FEBS Lett 373, 245-9.
2. Grunewald, T.G. and Butt, E. (2008) Mol Cancer 7, 31.
3. Schreiber, V. et al. (1998) Mol Med 4, 675-87.
4. Chew, C.S. et al. (2002) J Cell Sci 115, 4787-99.
5. Rachlin, A.S. and Otey, C.A. (2006) J Cell Sci 119, 995-1004.
6. Chew, C.S. et al. (2000) J Cell Sci 113 ( Pt 11), 2035-45.
7. Lin, Y.H. et al. (2004) J Cell Biol 165, 421-32.
8. Grunewald, T.G. et al. (2007) Br J Cancer 96, 296-305.
9. Frietsch, J.J. et al. (2010) Br J Cancer 102, 1645-53.
10. Traenka, C. et al. (2010) Cancer Res 70, 8003-14.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

LASP1 Antibody detects endogeneous levels of total LASP1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala191 of human LASP1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

LASP1 is a cytoskeletal scaffold protein belonging to the LIM protein subfamily (1,2). LASP1 consists of an N-terminal LIM domain, followed by two nebulin repeats, and a C-terminal SH3 domain (1,3). The nebulin repeats interact with actin, while the SH3 domain interacts with palladin (4,5), suggesting LASP1 functions as an actin-binding protein, possibly in cytoskeletal organization. LASP1 has been shown to localize to focal adhesions, lamellipodia, and membrane ruffles (6-8) and might be involved in membrane migration. Overexpression of LASP1 has been associated with metastatic cancers, such as breast and ovarian cancer (2). In these cases, membrane, cytoplasmic, and nuclear localization of LASP1 in the tumor cell has been reported, suggesting LASP1 involvement in membrane and nuclear signaling (9,10).

1. Tomasetto, C. et al. (1995) FEBS Lett 373, 245-9.
2. Grunewald, T.G. and Butt, E. (2008) Mol Cancer 7, 31.
3. Schreiber, V. et al. (1998) Mol Med 4, 675-87.
4. Chew, C.S. et al. (2002) J Cell Sci 115, 4787-99.
5. Rachlin, A.S. and Otey, C.A. (2006) J Cell Sci 119, 995-1004.
6. Chew, C.S. et al. (2000) J Cell Sci 113 ( Pt 11), 2035-45.
7. Lin, Y.H. et al. (2004) J Cell Biol 165, 421-32.
8. Grunewald, T.G. et al. (2007) Br J Cancer 96, 296-305.
9. Frietsch, J.J. et al. (2010) Br J Cancer 102, 1645-53.
10. Traenka, C. et al. (2010) Cancer Res 70, 8003-14.

注意事项：

Storage: Supplied in 10mM HEPES (pH 7.5), 150 mM NaCl,
100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot
the antibody.