Lamin A/C (4C11) Mouse mAb (Alexa Fluor? 488 Conjugate)

• 产品编号：CST-8617S      品牌：CST       原厂货号：8617S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Lamin A/C (4C11) Mouse mAb (Alexa Fluor® 488 Conjugate) 鼠单抗能够检测内源的lamin A 和C 蛋白。它也能检测在凋亡过程中被caspase 裂解的大片段lamin A (50 kDa)和lamin C (41 kDa) 。此抗体不与lamins B1和B2交叉反应。该单克隆抗体是通过重组的人 lamin A 蛋白免疫动物而获得。该 CST 抗体与Alexa Fluor® 488荧光染料偶联，并且在公司内部通过用人源细胞进行流式细胞术实验进行了证实。此抗体预计与未偶联的抗体Lamin A/C (4C11) Mouse mAb #4777具有同源交叉反应。Lamin是核膜的结构组分，对维持正常的细胞功能非常重要，比如细胞周期调控、DNA修复和染色质组装（1-3）。Lamin A/C能够被caspase-6切割，可以作为caspase-6激活的标志。在凋亡过程中，Lamin A/C能够被特异地剪切成大片段(41-50 kDa)和小片段 (28 kDa)（3,4）。Lamin的剪切会引起细胞核的失调和细胞死亡（5，6）。

1. Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
2. Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84.
3. Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
4. Orth, K. et al. (1996) J Biol Chem 271, 16443-6.
5. Oberhammer, F.A. et al. (1994) J Cell Biol 126, 827-37.
6. Rao, L. et al. (1996) J Cell Biol 135, 1441-55.

原厂资料：

Specificity / Sensitivity

Lamin A/C (4C11) Mouse mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of lamin A and C proteins. It also reacts with the larger fragments of lamin A (50 kDa) and lamin C (41 kDa) produced by caspase cleavage during apoptosis. This antibody does not cross-react with lamins B1 and B2.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant fragment of human lamin A protein.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Lamin A/C (4C11) Mouse mAb #4777.

Background

Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear disregulation and cell death (5,6).

1. Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
2. Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84.
3. Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
4. Orth, K. et al. (1996) J Biol Chem 271, 16443-6.
5. Oberhammer, F.A. et al. (1994) J Cell Biol 126, 827-37.
6. Rao, L. et al. (1996) J Cell Biol 135, 1441-55.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4ºC. Protect from light. Do not
freeze.