Cytoskeletal Marker Antibody Sampler Kit

• 产品编号：CST-8614S      品牌：Cell Signaling Technology       原厂货号：8614S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

Desmin (D93F5) XP®兔mAb, Keratin 17 (D73C7) XP®兔mAb, β-Tubulin (9F3) Rabbit mAb和Vimentin (D21H3) XP® Rabbit mAb以及B-Actin (D68) Rabbit mAb可以分别识别内源性的靶蛋白。Pan-Keratin (C11)mouse mAb可以识别内源性总的keratins 4, 5, 6, 8, 10, 13, 和 18蛋白。抗体与其他keratins没有交叉反应。兔单克隆抗体由合成肽段免疫动物产生，该肽段分别与人β-actin氨基端，人desmin蛋白羧基端，人kerain17蛋白羧基端，人β-tubulin氨基端或人vimentin蛋白45位精氨酸附近残基序列一致。小鼠单克隆抗体使用A-431细胞的细胞骨架免疫动物获得。Cytoskeletal Marker Antibody Sampler试剂盒提供了一种经济的方式检测细胞骨架相关蛋白的存在和状态。试剂盒含有足以进行4次western blot实验的一抗。细胞骨架由3种不同的细胞纤维组成：微管，微丝（actin）和中间丝。Actin，一种广泛表达的真核蛋白，是细胞骨架的主要成分。哺乳动物中至少发现了6种亚型。非肌肉β- 和γ-actin，也被称为细胞质actin，主要表达在非肌肉细胞中，控制细胞结构和运动（1）。中间丝的主要类型是部分通过其组织特异性决定的，例如：cytokeratins（上皮细胞），vimentin（间充质来源）和desmin（骨骼，内脏和某些血管平滑肌细胞）（2）。角蛋白的异二聚体由一个酸性角蛋白（或type I角蛋白，角蛋白9-23）和一个碱性角蛋白（或type II角蛋白，角蛋白1-8）聚合形成中间丝（3）。研究表明vimentin在肉瘤中表达，而在肿瘤中不表达，它的表达与其他标记蛋白相结合用于研究区分这二种瘤（4）。Desmin是发育早期的成肌标志，它会与filaments形成网络以横跨肌原纤维并围绕Z盘（5）。α/β-tubulin异二聚体形成微管亚基组成microtubule building block（6）。 


原厂资料：

Specificity / Sensitivity

Desmin (D93F5) XP® Rabbit mAb, Keratin 17 (D73C7) XP® Rabbit mAb, β-Tubulin (9F3) Rabbit mAb, and Vimentin (D21H3) XP® Rabbit mAb, and B-Actin (D68) Rabbit mAb recognize endogenous levels of total respective target proteins. Pan-Keratin (C11) Mouse mAb detects endogenous levels of total keratins 4, 5, 6, 8, 10, 13, and 18. This antibody does not cross-react with other keratins.

Source / Purification

Rabbit monoclonal antibodies are produced by immunizing animals with a synthetic peptide cooresponding to residues near the amino terminus of human β-actin protein, carboxy terminal residues of human desmin protein, residues near the carboxy terminus of human keratin 17 protein, the amino terminus of human β-tubulin protein, or residues surrounding Arg45 of human vimentin protein. Mouse monoclonal antibody is produced by immunizing animals with a cytoskeleton preparation from A-431 cells.

Description

The Cytoskeletal Marker Antibody Sampler Kit provides an economical means to evaluate the presence and status of select cytoskeleton associated proteins. The kit provides enough primary antibodies to perform four western blots per primary antibody.

Background

The cytoskeleton consists of three different types of cystosolic fibers: microtubules, microfilaments (actin) and intermediate filaments. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). Major types of intermediate filaments are distinguished in part by the tissue in which they are expressed, for example; cytokeratins (epithelial cells), vimentin (mesenchyme origin), and desmin (skeletal, visceral and certain vascular smooth muscle cells) (2). Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form intermediate filaments (3). Research studies have demonstrated that vimentin is present in sarcomas, but not carcinomas, and its expression is examined relative to other markers in order to distinguish between the two forms of neoplasm (4). Desmin is a myogenic marker expressed in early development that forms a network of filaments that extends across the myofibril and surrounds Z discs (5). α/β-tubulin heterodimers form the tubulin subunit that comprises the microtubule building block (6).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.