CA2 Antibody

• 产品编号：CST-8612S      品牌：CST       原厂货号：8612S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CA2 Antibody用于检测内源性CA2总蛋白水平。该多抗通过用与人源CA2蛋白Lys169位点周围残基序列相同的合成肽免疫动物制备而得。抗体的纯化采用蛋白A与肽段亲和层析相结合的方法。碳酸酐酶(CA)是一种古老的锌金属酶家族，存在于几乎所有的生物中。CA可以分成三个大类(α, β, 和γ)，它们是独立进化的，并且在序列和总体折叠方式上也有很大不同。功能性的CA在它的活性位点都有一个锌原子，这对于它的催化作用是必需的，它在CO2水化形成HCO3-和H+的化学反应中起着可逆的催化作用。哺乳类的CA有很多种的同工酶，它们都隶属α类CA(1,2)。

1. Smith, K.S. et al. (1999) Proc Natl Acad Sci USA 96, 15184-9.
2. Tripp, B.C. et al. (2001) J Biol Chem 276, 48615-8.
3. McMahon, C. et al. (2001) Blood 97, 1947-50.
4. Borthwick, K.J. et al. (2003) J Med Genet 40, 115-21.
5. Lai, L.W. et al. (1998) J Clin Invest 101, 1320-5.
6. Palminiello, S. et al. (2008) Brain Res 1190, 193-205.
7. Jang, B.G. et al. (2010) J Alzheimers Dis 21, 939-45.
8. Parkkila, S. et al. (2010) Mod Pathol 23, 743-50.
9. Becker, H.M. et al. (2005) J Biol Chem 280, 39882-9.
10. Becker, H.M. et al. (2011) Proc Natl Acad Sci USA 108, 3071-6.

原厂资料：

Specificity / Sensitivity

CA2 Antibody recognizes endogenous levels of total CA2 protein.

Background

Carbonic anhydrases (CA) are a family of ancient zinc metalloenzymes found in almost all living organisms. All CA can be divided into 3 distinct classes (α, β, and γ) that evolved independently and have no significant homology in sequence and overall folding. All functional CA catalyze the reversible hydration of CO2 into HCO3- and H+ and contain a zinc atom in the active sites essential for catalysis. There are many isoforms of CA in mammals and they all belong to the α class (1,2).

1. Smith, K.S. et al. (1999) Proc Natl Acad Sci USA 96, 15184-9.
2. Tripp, B.C. et al. (2001) J Biol Chem 276, 48615-8.
3. McMahon, C. et al. (2001) Blood 97, 1947-50.
4. Borthwick, K.J. et al. (2003) J Med Genet 40, 115-21.
5. Lai, L.W. et al. (1998) J Clin Invest 101, 1320-5.
6. Palminiello, S. et al. (2008) Brain Res 1190, 193-205.
7. Jang, B.G. et al. (2010) J Alzheimers Dis 21, 939-45.
8. Parkkila, S. et al. (2010) Mod Pathol 23, 743-50.
9. Becker, H.M. et al. (2005) J Biol Chem 280, 39882-9.
10. Becker, H.M. et al. (2011) Proc Natl Acad Sci USA 108, 3071-6.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.