TBP Antibody

• 产品编号：CST-8515S      品牌：CST       原厂货号：8515S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

TBP Antibody能够检测内源性TBP总蛋白水平。该多克隆抗体是采用合成的与人源TBP蛋白Gln44残基周围序列相对应的肽段免疫动物而生产的。通过蛋白A和多肽亲和层析纯化抗体获得。TATA-binding protein (TBP)是一个广泛表达的细胞核蛋白，该蛋白在一般转录因子蛋白复合物TFIID中心起作用(1-3)。TFIID包含TBP和13 TBP相关因子(TAFs)，该蛋白有助于转录前起始复合物的形成，该复合物是由多种蛋白复合物 (TFIIA, TFIIB, TFIIE, TFIIF, TFIIH, and RNA polymerase II)装配，在转录的起始阶段这些蛋白质结合到一个基因启动子上(1-3)。一旦前起始复合物形成，RNA polymerase II有助于延伸和基因转录。TBP通过结合到TATA-box序列从而发挥TFIID的招募功能，TATA-box序列发现在许多蛋白质编码基因的转录起始位点上游的大约25碱基对。因此，许多转录激活蛋白与TBP和多种TAF蛋白相互作用，并且有助于TFIID的招募和前起始复合物的形成。

1. Goodrich, J.A. and Tjian, R. (1994) Curr Opin Cell Biol 6, 403-9.
2. Berk, A.J. (2000) Cell 103, 5-8.
3. Thomas, M.C. and Chiang, C.M. (2006) Crit Rev Biochem Mol Biol 41, 105-78.

原厂资料：

Specificity / Sensitivity

TBP Antibody recognizes endogenous levels of total TBP protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln44 of human TBP protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

TATA-binding protein (TBP) is a ubiquitously expressed nuclear protein that functions at the core of the general transcription factor protein complex TFIID (1-3). TFIID, which contains TBP and 13 TBP-associated factors (TAFs), contributes to the formation of the transcription pre-initiation complex, an assembly of multiple protein complexes (TFIIA, TFIIB, TFIIE, TFIIF, TFIIH, and RNA polymerase II) that bind to a gene promoter during the initiation of transcription (1-3). Once the pre-initiation complex is formed, RNA polymerase II becomes competent for elongation and transcribes the body of a gene. TBP functions in the recruitment of TFIID by binding to the TATA-box sequence found approximately 25 base pairs upstream of the transcription start site of many protein-coding genes. In addition, many transcriptional activator proteins interact with TBP and various TAF proteins to facilitate recruitment of TFIID and formation of the pre-initiation complex.

1. Goodrich, J.A. and Tjian, R. (1994) Curr Opin Cell Biol 6, 403-9.
2. Berk, A.J. (2000) Cell 103, 5-8.
3. Thomas, M.C. and Chiang, C.M. (2006) Crit Rev Biochem Mol Biol 41, 105-78.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody