FLIP (D16A8) Rabbit mAb

• 产品编号：CST-8510S      品牌：CST       原厂货号：8510S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

FLIP (D16A8) Rabbit mAb兔单抗能够检测内源性水平的FLIP总蛋白。该抗体也能检测FLIP的短亚型，FLIPS。该抗体能够识别某未知来源大小为70 kDa的蛋白。该单克隆抗体是采用合成的人源FLIP蛋白组氨酸(160位)周围残基相对应的肽段免疫动物而制备的。细胞的FLIP（FLICE抑制蛋白）是一种细胞凋亡调节子，其有很多种名称，如c-FLIP (1)、Casper (2)、CLARP (3)、FLAME (4)、I-FLICE (5)、MRIT (6)、CASH (7)和Usurpin (8)。FLIP以两种剪接形式选择性表达， 即短FLIP（FLIPS）和长FLIP（FLIPL）。FLIPS包含两个死亡效应域（DEDS），发现存在于死亡受体衔接蛋白FADD和caspase-8前体域。FLIPL与caspase-8（FLICE）共享重要的同源域，并包含一个额外的死亡效应域，但FLIPL缺乏半胱天冬酶的催化活性位点，并且不具有蛋白酶活性。据研究报道，这两种形式都与FADD和caspase-8前体相互作用。关于FLIP在凋亡中的作用的研究是有争议性的，一些报道是抗细胞凋亡，而另一些则是促凋亡。FLIPL的过表达可导致caspase-8异源化从而产生活性蛋白酶，进而导致细胞凋亡。然而在生理水平，研究认为FLIP结合到FADD的DED会抑制caspase-8。通过siRNA或基因打靶降低FLIP表达，会使细胞对死亡受体介导的细胞凋亡敏感。FLIP也与癌细胞凋亡的阻滞相关，在某些类型的癌症包括Hodgkin's 淋巴瘤、卵巢癌和结肠癌中上调表达（9）。

1. Irmler, M. et al. (1997) Nature 388, 190-195.
2. Shu, H. B. et al. (1997) Immunity 6, 751-763.
3. Inohara, N. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 10717-10722.
4. Srinivasula, S. M. et al. (1997) J. Biol. Chem. 272, 18542-18545.
5. Hu, S. et al. (1997) J. Biol. Chem. 272, 17255-17257.
6. Han, D. K. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 11333-11338.
7. Dita, R. M. et al. (1998) Cell Death Differ. 5, 271-288.
8. Goltsev, Y. V. et al. (1997) J. Biol. Chem. 272, 19641-19644.
9. Kataoka, T. (2005) Crit. Rev. Immunol. 25, 31-58.

原厂资料：

Specificity / Sensitivity

FLIP (D16A8) Rabbit mAb recognizes endogenous levels of total FLIP protein. This antibody also detects the short isoform of FLIP, FLIPS. The antibody detects a band of unknown origin 
 at around 70 kDa.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His160 of human FLIP protein.

Background

Cellular FLIP (FLICE inhibitory protein) is a regulator of apoptosis identified in different groups and has various names such as c-FLIP (1), Casper (2), CLARP (3), FLAME (4), I-FLICE (5), MRIT (6), CASH (7), and Usurpin (8). FLIP is expressed as two alternative splice forms, FLIP short (FLIPS) and FLIP long (FLIPL). FLIPS contains two death effector domains (DEDs) found on the death receptor adaptor protein FADD and the pro-domain of caspase-8. FLIPL shares significant homology with caspase-8 (FLICE) and contains an additional death effector domain, but FLIPL lacks the catalytic active site of the caspases and does not have protease activity. Both forms have been reported to interact with FADD and pro-caspase-8. The role of FLIP in apoptosis is controversial as some have reported it to be anti-apoptotic, while others claim that it is pro-apoptotic. Overexpression of FLIPL can lead to caspase-8 heterodimers that produce an active protease resulting in apoptosis. However, at physiological levels, it is thought that the binding of FLIP to the DED of FADD results in inhibition of caspase-8 processing. Reduction of FLIP by siRNA or gene targeting sensitizes cells to death receptor-mediated apoptosis. FLIP has also been implicated in the resistance of cancer cells to apoptosis and is upregulated in some cancer types including Hodgkin's lymphoma, and ovarian and colon carcinomas (9).

1. Irmler, M. et al. (1997) Nature 388, 190-195.
2. Shu, H. B. et al. (1997) Immunity 6, 751-763.
3. Inohara, N. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 10717-10722.
4. Srinivasula, S. M. et al. (1997) J. Biol. Chem. 272, 18542-18545.
5. Hu, S. et al. (1997) J. Biol. Chem. 272, 17255-17257.
6. Han, D. K. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 11333-11338.
7. Dita, R. M. et al. (1998) Cell Death Differ. 5, 271-288.
8. Goltsev, Y. V. et al. (1997) J. Biol. Chem. 272, 19641-19644.
9. Kataoka, T. (2005) Crit. Rev. Immunol. 25, 31-58.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody